Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China

doi:10.3390/ijms12095698

	Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China
	Zhang, Le 1,2; Sun, Weibang1 ; Wang, Zhonglang 1; Guan, Kaiyun 1; Yang, Junbo 3
通讯作者	Sun, WB (reprint author), Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Peoples R China
	2011-09-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	12 期号:9 页码:5698-5704
摘要	Hibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species.
关键词	Hibiscus Aridicola Endangered Plant Ssr Markers Population Structure Population Genetics
学科领域	Chemistry
DOI	10.3390/ijms12095698
收录类别	SCI
语种	英语
WOS记录号	WOS:000295213600019
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/5231
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Le,Sun, Weibang,Wang, Zhonglang,et al. Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(9):5698-5704.
APA	Zhang, Le,Sun, Weibang,Wang, Zhonglang,Guan, Kaiyun,&Yang, Junbo.(2011).Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(9),5698-5704.
MLA	Zhang, Le,et al."Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.9(2011):5698-5704.

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