Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China
Zhang, Le1,2; Sun, Weibang1; Wang, Zhonglang1; Guan, Kaiyun1; Yang, Junbo3
2011-09-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号12期号:9页码:5698-5704
摘要Hibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species.
关键词Hibiscus Aridicola Endangered Plant Ssr Markers Population Structure Population Genetics
资助信息China's Yunnan Government for qualified scientists and technicians[2006PY-48]; Botanic Gardens Conservation International (BGCI)[6206/R4331]; National Natural Science Foundation of China[30970192]
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/5231
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog, Kunming 650204, Peoples R China
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Zhang, Le,Sun, Weibang,Wang, Zhonglang,et al. Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(9):5698-5704.
APA Zhang, Le,Sun, Weibang,Wang, Zhonglang,Guan, Kaiyun,&Yang, Junbo.(2011).Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(9),5698-5704.
MLA Zhang, Le,et al."Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.9(2011):5698-5704.
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