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题名: New Sweet-Tasting Oleanane-Type Triterpenoid Saponins from "Tugancao" (Derris eriocarpa How)
作者: Zhang, Hong-Xia1; Sun, Guo1, 2; Gu, Jian-Long3; Du, Zhi-Zhi1
刊名: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
关键词: Derris eriocarpa ; sweet-tasting ; triterpenoid saponins ; sensory evaluation ; acute toxicity
英文摘要: With the aim to investigate the sweet-tasting compounds in Derris eriocarpa How (a substitute for licorice in "Zhuang" and "Dai" ethnopharmacy in Guangxi and Yunnan provinces of China) as well as to ascertain why the stem of D. eriocarpa can be used to substitute for licorice in the sweetness taste aspect, taste sensory-guided fractionation was conducted to isolate sweet constituents from the extract of D. eriocarpa: Pour sweet-tasting tritdrpenoid saponins were obtained, including millettiasaponin A (1) and three new saponins named derrisaponins A-C (2-4). The sweetness potency was evaluated by a human sensory panel test. The sweetness intensities of compounds 1-4 were determined to be approximately 150, 80, 2, and 0.5 times relative to sucrose at the concentration of 1%, respectively, of which compounds 1 and 2, with a free carboxyl group at the C-30 position, showed more potent sweetness intensity. In addition, compounds 1 and 2 showed no acute toxic activity at doses of 250 and 400 mg/kg of body weight, respectively, assessed through caudal vein injection to ICR mice. The contents of the sweetest compounds in stems were analyzed quantitatively as 352.80 mg/kg for compound 1 and 1887.60 mg/kg for compound 2 performed by ultra-performance liquid chromatography tandem mass spectrometry.
出版日期: 2017-03-22
卷号: 65, 期号:11, 页码:2357-2363
DOI标识: 10.1021/acs.jafc.7b00137
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/51008
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yunnan Tobacco Qual Inspect Supervis Stn, Kunming 650106, Yunnan, Peoples R China

Recommended Citation:
Zhang, Hong-Xia,Sun, Guo,Gu, Jian-Long,et al. New Sweet-Tasting Oleanane-Type Triterpenoid Saponins from "Tugancao" (Derris eriocarpa How)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(11):2357-2363.
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