| Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi | |
Yang, Yan-long1 ; Zhang, Shasha3; Ma, Ke1,2; Xu, Yuxing4; Tao, Qiaoqiao1,2; Chen, Yihua5; Chen, Juan6,7; Guo, Shunxing6,7; Ren, Jinwei1; Wang, Wenzhao1; Tao, Yong3; Yin, Wen-Bing1,2; Liu, Hongwei1,2
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| 2017-04-18 | |
| 发表期刊 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
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| 卷号 | 56期号:17页码:4749-4752 |
| 摘要 | Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining using the EriG protein sequence as a probe led to the discovery of a new family of ubiquitous UbiA-related diterpene cyclases in bacteria and fungi. We successfully characterized seven new diterpene cyclases from bacteria or basidiomycete fungi with the help of an engineered Escherichia coli strain and determined the structures of their corresponding products. A new diterpene with an unusual skeleton was generated during this process. The discovery of this new family of diterpene cyclases provides new insight into the UbiA superfamily. |
| 关键词 | Biosynthesis Cyclases Diterpenes Natural Products Ubia |
| DOI | 10.1002/anie.201700565 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000398576000010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/50995 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Mycol, Inst Microbiol, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 5.Chinese Acad Sci, State Key Lab Microbial Resources, Yunnan Inst Microbiol, Beijing 100101, Peoples R China 6.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China 7.Peking Union Med Coll, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yan-long,Zhang, Shasha,Ma, Ke,et al. Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(17):4749-4752. |
| APA | Yang, Yan-long.,Zhang, Shasha.,Ma, Ke.,Xu, Yuxing.,Tao, Qiaoqiao.,...&Liu, Hongwei.(2017).Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(17),4749-4752. |
| MLA | Yang, Yan-long,et al."Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.17(2017):4749-4752. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| anie201700565.pdf（2077KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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