| Isolation and characterization of cytotoxic cyclotides from Viola tricolor | |
| Tang, Jun1,3; Wang, Conan K.2; Pan, Xulin1; Yan, He1,3; Zeng, Guangzhi1; Xu, Wenyan1,3; He, Wenjun1,3; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1 | |
| 2010-08-01 | |
| 发表期刊 | PEPTIDES
 |
| ISSN | 0196-9781 |
| 卷号 | 31期号:8页码:1434-1440 |
| 摘要 | Many plants of the Violaceae plant family have been used in traditional remedies, and these plants often contain cyclotides, a particular type of plant cyclopeptide that is distinguished by a cyclic cystine knot motif. In general, bioactive plant cyclopeptides are interesting candidates for drug development. In the current study, a suite of 14 cyclotides, which includes seven novel cyclotides [vitri B, C, D, E, F, vary Hm, and He], together with seven known cyclotides [vary A, D, E, F, H, vitri A, and cycloviolacin O2], was isolated from Viola tricolor, a common flower. A chromatography-based method was used to isolate the cyclotides, which were characterized using tandem mass spectrometry and NMR spectroscopy. Several of the cyclotides showed cytotoxic activities against five cancer cell lines, U251, MDA-MB-231, A549, DU145, and BEL-7402. Three cyclotides, vitri A, vitri F, and cycloviolacin O2, were the most cytotoxic. The cytotoxic activity of the cyclotides did not correlate well with their hemolytic activity, indicating that different interactions, most likely with membranes, are involved for cytotoxic and hemolytic activities. Homology modeling of the structures was used in deriving structure-activity relationships. (C) 2010 Elsevier Inc. All rights reserved. |
| 关键词 | Cyclotides Viola Tricolor Cytotoxic Activity Vitri B-f Varv Hm Varv He |
| 学科领域 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| DOI | 10.1016/j.peptides.2010.05.004 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280663600003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/4868 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Jun,Wang, Conan K.,Pan, Xulin,et al. Isolation and characterization of cytotoxic cyclotides from Viola tricolor[J]. PEPTIDES,2010,31(8):1434-1440. |
| APA | Tang, Jun.,Wang, Conan K..,Pan, Xulin.,Yan, He.,Zeng, Guangzhi.,...&Tan, Ninghua.(2010).Isolation and characterization of cytotoxic cyclotides from Viola tricolor.PEPTIDES,31(8),1434-1440. |
| MLA | Tang, Jun,et al."Isolation and characterization of cytotoxic cyclotides from Viola tricolor".PEPTIDES 31.8(2010):1434-1440. |
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