Isolation and characterization of cytotoxic cyclotides from Viola tricolor
Tang, Jun1,3; Wang, Conan K.2; Pan, Xulin1; Yan, He1,3; Zeng, Guangzhi1; Xu, Wenyan1,3; He, Wenjun1,3; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1
2010-08-01
发表期刊PEPTIDES
ISSN0196-9781
卷号31期号:8页码:1434-1440
摘要Many plants of the Violaceae plant family have been used in traditional remedies, and these plants often contain cyclotides, a particular type of plant cyclopeptide that is distinguished by a cyclic cystine knot motif. In general, bioactive plant cyclopeptides are interesting candidates for drug development. In the current study, a suite of 14 cyclotides, which includes seven novel cyclotides [vitri B, C, D, E, F, vary Hm, and He], together with seven known cyclotides [vary A, D, E, F, H, vitri A, and cycloviolacin O2], was isolated from Viola tricolor, a common flower. A chromatography-based method was used to isolate the cyclotides, which were characterized using tandem mass spectrometry and NMR spectroscopy. Several of the cyclotides showed cytotoxic activities against five cancer cell lines, U251, MDA-MB-231, A549, DU145, and BEL-7402. Three cyclotides, vitri A, vitri F, and cycloviolacin O2, were the most cytotoxic. The cytotoxic activity of the cyclotides did not correlate well with their hemolytic activity, indicating that different interactions, most likely with membranes, are involved for cytotoxic and hemolytic activities. Homology modeling of the structures was used in deriving structure-activity relationships. (C) 2010 Elsevier Inc. All rights reserved.
关键词Cyclotides Viola Tricolor Cytotoxic Activity Vitri B-f Varv Hm Varv He
资助信息NSFC ; ARC ; National Natural Science Foundation of China [30725048]; National Basic Research Program of China [2009 CB522300]; Science and Technology Department of Yunnan Province [2008OC011]; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences
学科领域Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
DOI10.1016/j.peptides.2010.05.004
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS类目Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS记录号WOS:000280663600003
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/4868
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Tang, Jun,Wang, Conan K.,Pan, Xulin,et al. Isolation and characterization of cytotoxic cyclotides from Viola tricolor[J]. PEPTIDES,2010,31(8):1434-1440.
APA Tang, Jun.,Wang, Conan K..,Pan, Xulin.,Yan, He.,Zeng, Guangzhi.,...&Tan, Ninghua.(2010).Isolation and characterization of cytotoxic cyclotides from Viola tricolor.PEPTIDES,31(8),1434-1440.
MLA Tang, Jun,et al."Isolation and characterization of cytotoxic cyclotides from Viola tricolor".PEPTIDES 31.8(2010):1434-1440.
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