Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids

doi:10.1021/np800657j

	Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids
	Zhao, Pei-Ji; Gao, Suo; Fan, Li-Ming; Nie, Jing-Lei; He, Hong-Ping; Zeng, Ying; Shen, Yue-Mao; Hao, Xiao-Jiang
	2009-04-01
发表期刊	JOURNAL OF NATURAL PRODUCTS
ISSN	0163-3864
卷号	72 期号:4 页码:645-649
摘要	To determine the biosynthesis pathway of the atisine-type diterpenoid alkaloids spiramines A/B and C/D, feeding experiments in in vitro Cultured plantlets; and enzymatic transformations in cell-free extracts were performed in combination with LCMS and tandem MS analyses. L-[2-(13)C,(15)N]Serine was used in the feeding experiments and enzymatic transformations, and the diterpene spiraminol was identified as it biosynthetic precursor of spiramine alkaloids. The LCMS and tandem MS spectra of the extracts from these experiments indicated that L-[2-(13)C,(15)N]serine was incorporated into spiramines A/B and C/D. The labeled reaction products show that L-serine is the one possible nitrogen source involved in the biosynthesis of atisine-type DAs.
关键词	Rubescens Var.-taihangensis Rabdosia-pseudo-irrorata Kaurene Diterpenoids
学科领域	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI	10.1021/np800657j
收录类别	SCI ; IC
语种	英语
WOS记录号	WOS:000265530000008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/4816
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Pei-Ji,Gao, Suo,Fan, Li-Ming,et al. Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids[J]. JOURNAL OF NATURAL PRODUCTS,2009,72(4):645-649.
APA	Zhao, Pei-Ji.,Gao, Suo.,Fan, Li-Ming.,Nie, Jing-Lei.,He, Hong-Ping.,...&Hao, Xiao-Jiang.(2009).Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids.JOURNAL OF NATURAL PRODUCTS,72(4),645-649.
MLA	Zhao, Pei-Ji,et al."Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids".JOURNAL OF NATURAL PRODUCTS 72.4(2009):645-649.

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