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题名: Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids
作者: Zhao, Pei-Ji1; Gao, Suo1; Fan, Li-Ming1; Nie, Jing-Lei1; He, Hong-Ping1; Ying ZENG(曾英)1; Shen, Yue-Mao1; null(郝小江)1
刊名: JOURNAL OF NATURAL PRODUCTS
关键词: RUBESCENS VAR.-TAIHANGENSIS ; RABDOSIA-PSEUDO-IRRORATA ; KAURENE DITERPENOIDS
英文摘要: To determine the biosynthesis pathway of the atisine-type diterpenoid alkaloids spiramines A/B and C/D, feeding experiments in in vitro Cultured plantlets; and enzymatic transformations in cell-free extracts were performed in combination with LCMS and tandem MS analyses. L-[2-(13)C,(15)N]Serine was used in the feeding experiments and enzymatic transformations, and the diterpene spiraminol was identified as it biosynthetic precursor of spiramine alkaloids. The LCMS and tandem MS spectra of the extracts from these experiments indicated that L-[2-(13)C,(15)N]serine was incorporated into spiramines A/B and C/D. The labeled reaction products show that L-serine is the one possible nitrogen source involved in the biosynthesis of atisine-type DAs.
出版日期: 2009-04-01
卷号: 72, 期号:4, 页码:645-649
DOI标识: 10.1021/np800657j
语种: 英语
ISSN号: 0163-3864
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/4816
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China

Recommended Citation:
Zhao, PJ; Gao, S; Fan, LM; Nie, JL; He, HP; Zeng, Y; Shen, YM; Hao, XJ.Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids,JOURNAL OF NATURAL PRODUCTS,2009,72(4):645-649
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