Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages

doi:10.1021/np9001465

	Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages
	Aquila, Silvia 2; Weng, Zhi-Ying 3; Zeng, Yue-Qin 3; Sun, Han-Dong1 ; Luis Rios, Jose 2
	2009-07-01
发表期刊	JOURNAL OF NATURAL PRODUCTS
ISSN	0163-3864
卷号	72 期号:7 页码:1269-1272
摘要	Xerophilusin A (1), xerophilusin B (2), longikaurin B (3), and xerophilusin F (4) from Isodon xerophylus inhibit LPS-induced NO production in RAW 264.7 macrophages, with IC(50) values of 0.60, 0.23, 0.44, and 0.67 mu M, respectively. and they all inhibited mRNA production in these same cells. They decreased the luciferase activity in RAW 264.7 cells transiently transfected with the NF-kappa B-dependent luciferase reporter, with IC(50) values of 1.8, 0.7. 1.2, and 1.6 mu M, respectively. Compounds 1-3 reduced NF-kappa B activation, with compound 4 showing no effect, but p65 translocation from the cytoplasm to the nucleus and the LPS-induced degradation of 1 kappa B were inhibited by all four test Compounds. These findings indicate that ent-kauranes are potential anti-inflammatory agents, with a specific mechanism in which both the inhibition of NF-kappa B translocation and the consequent decrease of pro-inflammatory mediators are implicated.
关键词	Trijugin-type Limonoids Trichilia-connaroides Sandoricum-koetjape Carbon Skeleton Meliaceae Baccifera Fruticosa
学科领域	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI	10.1021/np9001465
收录类别	SCI
语种	英语
WOS记录号	WOS:000268664500009
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/4790
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 2.Univ Valencia, Fac Farm, Dept Farmacol, Valencia 46100, Spain 3.Kunming Med Coll, Dept Pharm, Kunming 650031, Peoples R China
推荐引用方式 GB/T 7714	Aquila, Silvia,Weng, Zhi-Ying,Zeng, Yue-Qin,et al. Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages[J]. JOURNAL OF NATURAL PRODUCTS,2009,72(7):1269-1272.
APA	Aquila, Silvia,Weng, Zhi-Ying,Zeng, Yue-Qin,Sun, Han-Dong,&Luis Rios, Jose.(2009).Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages.JOURNAL OF NATURAL PRODUCTS,72(7),1269-1272.
MLA	Aquila, Silvia,et al."Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages".JOURNAL OF NATURAL PRODUCTS 72.7(2009):1269-1272.

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