A geraniol-synthase gene from Cinnamomum tenuipilum
Yang, T; Li, J; Wang, HX; Zeng, Y
2005-02-01
发表期刊PHYTOCHEMISTRY
ISSN0031-9422
卷号66期号:3页码:285-293
摘要Geraniol may accumulate up to 86-98% of the leaf essential oils in geraniol chemotypes of the evergreen camphor tree Cinnamomum tenuipilum. A similarity-based cloning strategy yielded a cDNA clone that appeared to encode a terpene synthase and which could be phylogenetically grouped within the angiosperm monoterpene synthase/subfamily. After its expression in Escherichia coli and enzyme assay with prenyl diphosphates as substrates, the enzyme encoded by the putative C. tenuipilum monoterpene synthase gene was shown to specifically convert geranyl diphosphate to geraniol as a single product by GC-MS analysis. Biochemical characterization of the partially purified recombinant protein revealed a strong dependency for Mg2+ and Mn2+, and an apparent Michaelis constant of 55.8 muM for geranyl diphosphate. Thus, a new member of the monoterpene synthase family was identified and designated as CtGES. The genome contains a single copy of CtGES gene. Expression of CtGES was exclusively observed in the geraniol chemotype of C. tenuipilum. Furthermore, in situ hybridization analysis demonstrated that CtGES mRNA was localized in the oil cells of the leaves. (C) 2004 Elsevier Ltd. All rights reserved.
关键词Cinnamomum Tenuipilum Lauraceae Camphor Tree Functional Expression Chemotypes Essential Oil Ctges Gene Geraniol Synthase Monoterpene Synthases
学科领域Biochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.phytochem.2004.12.004
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号WOS:000227113100003
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被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/4722
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Yang, T,Li, J,Wang, HX,et al. A geraniol-synthase gene from Cinnamomum tenuipilum[J]. PHYTOCHEMISTRY,2005,66(3):285-293.
APA Yang, T,Li, J,Wang, HX,&Zeng, Y.(2005).A geraniol-synthase gene from Cinnamomum tenuipilum.PHYTOCHEMISTRY,66(3),285-293.
MLA Yang, T,et al."A geraniol-synthase gene from Cinnamomum tenuipilum".PHYTOCHEMISTRY 66.3(2005):285-293.
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