A geraniol-synthase gene from Cinnamomum tenuipilum
Yang, T; Li, J; Wang, HX; Zeng, Y
2005-02-01
Source PublicationPHYTOCHEMISTRY
ISSN0031-9422
Volume66Issue:3Pages:285-293
AbstractGeraniol may accumulate up to 86-98% of the leaf essential oils in geraniol chemotypes of the evergreen camphor tree Cinnamomum tenuipilum. A similarity-based cloning strategy yielded a cDNA clone that appeared to encode a terpene synthase and which could be phylogenetically grouped within the angiosperm monoterpene synthase/subfamily. After its expression in Escherichia coli and enzyme assay with prenyl diphosphates as substrates, the enzyme encoded by the putative C. tenuipilum monoterpene synthase gene was shown to specifically convert geranyl diphosphate to geraniol as a single product by GC-MS analysis. Biochemical characterization of the partially purified recombinant protein revealed a strong dependency for Mg2+ and Mn2+, and an apparent Michaelis constant of 55.8 muM for geranyl diphosphate. Thus, a new member of the monoterpene synthase family was identified and designated as CtGES. The genome contains a single copy of CtGES gene. Expression of CtGES was exclusively observed in the geraniol chemotype of C. tenuipilum. Furthermore, in situ hybridization analysis demonstrated that CtGES mRNA was localized in the oil cells of the leaves. (C) 2004 Elsevier Ltd. All rights reserved.
KeywordCinnamomum Tenuipilum Lauraceae Camphor Tree Functional Expression Chemotypes Essential Oil Ctges Gene Geraniol Synthase Monoterpene Synthases
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.phytochem.2004.12.004
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000227113100003
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/4722
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yang, T,Li, J,Wang, HX,et al. A geraniol-synthase gene from Cinnamomum tenuipilum[J]. PHYTOCHEMISTRY,2005,66(3):285-293.
APA Yang, T,Li, J,Wang, HX,&Zeng, Y.(2005).A geraniol-synthase gene from Cinnamomum tenuipilum.PHYTOCHEMISTRY,66(3),285-293.
MLA Yang, T,et al."A geraniol-synthase gene from Cinnamomum tenuipilum".PHYTOCHEMISTRY 66.3(2005):285-293.
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