| Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes | |
Gao, Lan1,2; Chen, Junling2,3; Gao, Jing2,3; Wang, Hongda3; Xiong, Wenyong1
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| 2017-01-15 | |
| 发表期刊 | JOURNAL OF CELL SCIENCE
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| 卷号 | 130期号:2页码:396-405 |
| 摘要 | GLUT4 (also known as SLC2A4) is essential for glucose uptake in skeletal muscles and adipocytes, which play central roles in whole-body glucose metabolism. Here, using direct stochastic optical reconstruction microscopy (dSTORM) to investigate the characteristics of plasma-membrane-fused GLUT4 at the single-molecule level, we have demonstrated that insulin and insulin resistance regulate the spatial organization of GLUT4 in adipocytes. Stimulation with insulin shifted the balance of GLUT4 on the plasma membrane toward a more dispersed configuration. In contrast, insulin resistance induced a more clustered distribution of GLUT4 and increased the mean number of molecules per cluster. Furthermore, our data demonstrate that the F(5)QQI motif and lipid rafts mediate the maintenance of GLUT4 clusters on the plasmamembrane. Mutation of F(5)QQI (F(5)QQA-GLUT4) induced a more clustered distribution of GLUT4; moreover, destruction of lipid rafts in adipocytes expressing F(5)QQA-GLUT4 dramatically decreased the percentage of large clusters and the mean number of molecules per cluster. In conclusion, our data clarify the effects of insulin stimulation or insulin resistance on GLUT4 reorganization on the plasma membrane and reveal new pathogenic mechanisms of insulin resistance. |
| 关键词 | Glut4 Insulin Resistance Direct Stochastic Optical Reconstruction Microscopy Dstorm Membrane Protein |
| DOI | 10.1242/jcs.192450 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394449000011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/46038 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanal Chem, Changchun 130022, Jilil, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Lan,Chen, Junling,Gao, Jing,et al. Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes[J]. JOURNAL OF CELL SCIENCE,2017,130(2):396-405. |
| APA | Gao, Lan,Chen, Junling,Gao, Jing,Wang, Hongda,&Xiong, Wenyong.(2017).Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes.JOURNAL OF CELL SCIENCE,130(2),396-405. |
| MLA | Gao, Lan,et al."Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes".JOURNAL OF CELL SCIENCE 130.2(2017):396-405. |
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| JCS192450supp.pdf（780KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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