中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 植物化学与西部植物资源持续利用国家重点实验室  > 期刊论文
题名: Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes
作者: Gao, Lan1, 2; Chen, Junling2, 3; Gao, Jing2, 3; Wang, Hongda3; Xiong, Wenyong1
刊名: JOURNAL OF CELL SCIENCE
出版日期: 2017-01-15
卷号: 130, 期号:2, 页码:396-405
关键词: GLUT4 ; Insulin resistance ; Direct stochastic optical reconstruction microscopy ; dSTORM ; Membrane protein
DOI: 10.1242/jcs.192450
文章类型: Article
英文摘要: GLUT4 (also known as SLC2A4) is essential for glucose uptake in skeletal muscles and adipocytes, which play central roles in whole-body glucose metabolism. Here, using direct stochastic optical reconstruction microscopy (dSTORM) to investigate the characteristics of plasma-membrane-fused GLUT4 at the single-molecule level, we have demonstrated that insulin and insulin resistance regulate the spatial organization of GLUT4 in adipocytes. Stimulation with insulin shifted the balance of GLUT4 on the plasma membrane toward a more dispersed configuration. In contrast, insulin resistance induced a more clustered distribution of GLUT4 and increased the mean number of molecules per cluster. Furthermore, our data demonstrate that the F(5)QQI motif and lipid rafts mediate the maintenance of GLUT4 clusters on the plasmamembrane. Mutation of F(5)QQI (F(5)QQA-GLUT4) induced a more clustered distribution of GLUT4; moreover, destruction of lipid rafts in adipocytes expressing F(5)QQA-GLUT4 dramatically decreased the percentage of large clusters and the mean number of molecules per cluster. In conclusion, our data clarify the effects of insulin stimulation or insulin resistance on GLUT4 reorganization on the plasma membrane and reveal new pathogenic mechanisms of insulin resistance.
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: PAIR CORRELATION-ANALYSIS ; PROTEIN HETEROGENEITY ; STORAGE-VESICLES ; CLATHRIN COATS ; ADIPOSE-CELLS ; LIPID RAFTS ; FUSION ; ADIPOCYTES ; LOCALIZATION ; PALM
收录类别: SCI
项目资助者: Chinese Academy of Sciences(292013312D11004) ; National Natural Science Foundation of China(81270919) ; Natural Science Foundation of Yunnan Province(39Y33H521261)
语种: 英语
WOS记录号: WOS:000394449000011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/46038
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
JCS192450supp.pdf(780KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanal Chem, Changchun 130022, Jilil, Peoples R China

Recommended Citation:
Gao, Lan,Chen, Junling,Gao, Jing,et al. Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes[J]. JOURNAL OF CELL SCIENCE,2017,130(2):396-405.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, Lan]'s Articles
[Chen, Junling]'s Articles
[Gao, Jing]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, Lan]‘s Articles
[Chen, Junling]‘s Articles
[Gao, Jing]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: JCS192450supp.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace