The anti-HIV-1 effect of scutellarin

doi:10.1016/j.bbrc.2005.06.166

	The anti-HIV-1 effect of scutellarin
	Zhang, GH ; Wang, Q; Chen, JJ; Zhang, XM; Tam, SC ; Zheng, YT
	2005-09-02
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	334 期号:3 页码:812-816
摘要	Scutellarin was purified from the plant Erigeron breuiscapus (Vant.) Hand.-Mazz. The activity against 3 strains of human immunodeficiency virus (HIV) was determined in vitro in this study. These were laboratory-derived virus (HIV-I-IIIB), drug-resistant virus (HIV-I-74V), and low-passage clinical isolated virus (HIV-1(KM018)). From syncytia inhibition study, the EC50 of scutellarin against HIV-I-IIB direct infection in C8166 cells was 26 mu M with a therapeutic index of 36. When the mode of infection changed from acute infection to cell-to-cell infection, this compound became even more potent and the EC50 reduced to 15 mu M. This suggested that cell fusion might be affected by this compound. By comparing the inhibitory effects on p24 antigen, scutellarin was also found to be active against HIV-1(74V) (EC50 253 mu M) and HIV-1(KM018) (EC50 136 mu M) infection with significant difference in potency. The mechanism of its action was also explored in this study. At a concentration of 433 mu M, scutellarin inhibited 48% of the cell free recombinant HIV-1 RT activity. It also caused 82% inhibition of HIV-1 particle attachment and 45% inhibition of fusion at the concentrations of 54 mu M. In summary, scutellarin was found to inhibit several strains of HIV-1 replication with different potencies. It appeared to inhibit HIV-1 RT activity, HIV-1 particle attachment and cell fusion. These are essential activities for viral transmission and replication. (c) 2005 Elsevier Inc. All rights reserved.
关键词	Scutellarin Hiv-1 Anti-hiv Agent Reverse Transcriptase Hiv Entry
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbrc.2005.06.166
收录类别	SCI
语种	英语
WOS记录号	WOS:000231226300011
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/4172
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Lab Mol Immunopharmacol, Kunming 650223, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan, Peoples R China 3.Chinese Univ Hong Kong, Dept Physiol, Hong Kong, Hong Kong, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, GH,Wang, Q,Chen, JJ,et al. The anti-HIV-1 effect of scutellarin[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,334(3):812-816.
APA	Zhang, GH,Wang, Q,Chen, JJ,Zhang, XM,Tam, SC,&Zheng, YT.(2005).The anti-HIV-1 effect of scutellarin.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,334(3),812-816.
MLA	Zhang, GH,et al."The anti-HIV-1 effect of scutellarin".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 334.3(2005):812-816.

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