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题名: Characterization and synthetic application of a novel beta 1,3-galactosyltransferase from Escherichia coli O55:H7
作者: Liu, Xian-wei1, 2, 3, 4; null(夏成峰)3, 4, 5; Li, Lei1, 2, 3, 4; Guan, Wan-yi1, 2, 3, 4; Pettit, Nicholas3, 4; Zhang, Hou-cheng1, 2; Chen, Min1, 2; Wang, Peng George3, 4
刊名: BIOORGANIC & MEDICINAL CHEMISTRY
关键词: O-Antigen, Galactosyltransferase ; Type 1 chain ; Lacto-N-tetraose
英文摘要: A beta 1,3-galactosyltransferase (WbgO) was identified in Escherichia coli O55:H7. Its function was confirmed by radioactive activity assay and structure analysis of the disaccharide synthesized with the recombinant enzyme. WbgO requires a divalent metal ion, either Mn2+ or Mg2+, for its activity and is active between pH 6.0-8.0 with a pH optimum of 7.0. N-acetylglucosamine (GlcNAc) and oligosaccharides with GlcNAc at the non-reducing end were shown to be its preferred substrates and it can be used for the synthesis of type 1 glycan chains from these substrates. Together with a recombinant bacterial GlcNAc-transferase, benzyl beta-lacto-N-tetraoside was synthesized with the purified WbgO to demonstrate the synthetic utility of WbgO. (C) 2009 Elsevier Ltd. All rights reserved.
出版日期: 2009-07-15
卷号: 17, 期号:14, 页码:4910-4915
DOI标识: 10.1016/j.bmc.2009.06.005
语种: 英语
ISSN号: 0968-0896
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/4154
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Shandong, Peoples R China
2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China
3.Ohio State Univ, Dept Chem, Columbus, OH 43202 USA
4.Ohio State Univ, Dept Biochem, Columbus, OH 43202 USA
5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China

Recommended Citation:
Liu, XW; Xia, CF; Li, L; Guan, WY; Pettit, N; Zhang, HC; Chen, M; Wang, PG.Characterization and synthetic application of a novel beta 1,3-galactosyltransferase from Escherichia coli O55:H7,BIOORGANIC & MEDICINAL CHEMISTRY,2009,17(14):4910-4915
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