Characterization and synthetic application of a novel beta 1,3-galactosyltransferase from Escherichia coli O55:H7
Liu, Xian-wei1,2,3,4; Xia, Chengfeng3,4,5; Li, Lei1,2,3,4; Guan, Wan-yi1,2,3,4; Pettit, Nicholas3,4; Zhang, Hou-cheng1,2; Chen, Min1,2; Wang, Peng George3,4
2009-07-15
发表期刊BIOORGANIC & MEDICINAL CHEMISTRY
ISSN0968-0896
卷号17期号:14页码:4910-4915
摘要A beta 1,3-galactosyltransferase (WbgO) was identified in Escherichia coli O55:H7. Its function was confirmed by radioactive activity assay and structure analysis of the disaccharide synthesized with the recombinant enzyme. WbgO requires a divalent metal ion, either Mn2+ or Mg2+, for its activity and is active between pH 6.0-8.0 with a pH optimum of 7.0. N-acetylglucosamine (GlcNAc) and oligosaccharides with GlcNAc at the non-reducing end were shown to be its preferred substrates and it can be used for the synthesis of type 1 glycan chains from these substrates. Together with a recombinant bacterial GlcNAc-transferase, benzyl beta-lacto-N-tetraoside was synthesized with the purified WbgO to demonstrate the synthetic utility of WbgO. (C) 2009 Elsevier Ltd. All rights reserved.
关键词Type 1 Chain O-antigen Lacto-n-tetraose Galactosyltransferase
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/4154
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Shandong, Peoples R China
2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China
3.Ohio State Univ, Dept Chem, Columbus, OH 43202 USA
4.Ohio State Univ, Dept Biochem, Columbus, OH 43202 USA
5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
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Liu, Xian-wei,Xia, Chengfeng,Li, Lei,et al. Characterization and synthetic application of a novel beta 1,3-galactosyltransferase from Escherichia coli O55:H7[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2009,17(14):4910-4915.
APA Liu, Xian-wei.,Xia, Chengfeng.,Li, Lei.,Guan, Wan-yi.,Pettit, Nicholas.,...&Wang, Peng George.(2009).Characterization and synthetic application of a novel beta 1,3-galactosyltransferase from Escherichia coli O55:H7.BIOORGANIC & MEDICINAL CHEMISTRY,17(14),4910-4915.
MLA Liu, Xian-wei,et al."Characterization and synthetic application of a novel beta 1,3-galactosyltransferase from Escherichia coli O55:H7".BIOORGANIC & MEDICINAL CHEMISTRY 17.14(2009):4910-4915.
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