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题名: Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants
作者: null(李德铢) ; null(高连明) ; Li, Hong-Tao ; null(王红) ; Ge, Xue-Jun ; Liu, Jian-Quan ; Chen, Zhi-Duan ; Zhou, Shi-Liang ; Chen, Shi-Lin ; Yang, Jun-Bo ; Fu, Cheng-Xin ; Zeng, Chun-Xia ; Yan, Hai-Fei ; Zhu, Ying-Jie ; Sun, Yong-Shuai ; Chen, Si-Yun ; Zhao, Lei ; Wang, Kun ; Yang, Tuo ; Duan, Guang-Wen
刊名: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
关键词: land plants ; species identification ; nuclear ribosomal (nr) DNA ; DNA BARCODES ; LAND PLANTS ; SPECIES IDENTIFICATION ; FLOWERING PLANTS ; CONSERVATION ; SPECIATION ; ITS2 ; EVOLUTIONARY ; PHYLOGENY ; ACCURACY
英文摘要: A two-marker combination of plastid rbcL and matK has previously been recommended as the core plant barcode, to be supplemented with additional markers such as plastid trnH-psbA and nuclear ribosomal internal transcribed spacer (ITS). To assess the effectiveness and universality of these barcode markers in seed plants, we sampled 6,286 individuals representing 1,757 species in 141 genera of 75 families (42 orders) by using four different methods of data analysis. These analyses indicate that (i) the three plastid markers showed high levels of universality (87.1-92.7%), whereas ITS performed relatively well (79%) in angiosperms but not so well in gymnosperms; (ii) in taxonomic groups for which direct sequencing of the marker is possible, ITS showed the highest discriminatory power of the four markers, and a combination of ITS and any plastid DNA marker was able to discriminate 69.9-79.1% of species, compared with only 49.7% with rbcL + matK; and (iii) where multiple individuals of a single species were tested, ascriptions based on ITS and plastid DNA barcodes were incongruent in some samples for 45.2% of the sampled genera (for genera with more than one species sampled). This finding highlights the importance of both sampling multiple individuals and using markers with different modes of inheritance. In cases where it is difficult to amplify and directly sequence ITS in its entirety, just using ITS2 is a useful backup because it is easier to amplify and sequence this subset of the marker. We therefore propose that ITS/ITS2 should be incorporated into the core barcode for seed plants.
出版日期: 2011
卷号: 108, 期号:49, 页码:19641-19646
语种: 英语
收录类别: sci
学科分类: Multidisciplinary Sciences
ISSN号: 0027-8424
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/3974
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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Li, De-Zhu; Gao, Lian-Ming; Li, Hong-Tao; Wang, Hong; Ge, Xue-Jun; Liu, Jian-Quan; Chen, Zhi-Duan; Zhou, Shi-Liang; Chen, Shi-Lin; Yang, Jun-Bo; Fu, Cheng-Xin; Zeng, Chun-Xia; Yan, Hai-Fei; Zhu, Ying-Jie; Sun, Yong-Shuai; Chen, Si-Yun; Zhao, Lei; Wang, Kun; Yang, Tuo; Duan, Guang-Wen.Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants,PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2011,108(49):19641-19646
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