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题名: Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region
作者: Yang, Ying1, 2; Zhai, Yanhong1, 2; Liu, Tao3; Zhang, Fangming1, 2; null(纪云恒)1
刊名: PLANTA MEDICA
关键词: Paris polyphylla var. chinensis ; Paris polyphylla var. yunnanensis ; Melanthiaceae ; Valeriana jatamansi ; Valerianaceae ; molecular authentication ; psbA-trnH
英文摘要: Two varieties of Paris polyphylla Smith (Melanthiaceae), P. polyphylla Smith var. chinensis (Franch.) Hara, and P. polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz., are used as medicinal Paris in China. Their dried rhizomes are the major source of raw material for some medicines. In recent years, medicinal Paris has been found to be adulterated with Valeriana jatamansi Jones (Valerianaceae) due to its high market demand and natural resource deficiency. After the chloroplast psbA-trnH regions of medicinal Paris and V. jatamansi were sequenced and analyzed, it was found that their characteristic sizes were > 1000 and around 250 bp, respectively. Based on length variation, medicinal Paris and the mixed adulterant were detected and distinguished from each other by amplification and electrophoresis. The amount of V. jatamansi that can be identified as an adulterant of medicinal Paris was also investigated. A trace amount (1 : 1000) of the adulterant was detected in the sensitivity tests. The established method has been proven to be sensitive and reliable.
出版日期: 2011
卷号: 77, 期号:1, 页码:87-91
语种: 英语
收录类别: SCI
学科分类: Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy
ISSN号: 0032-0943
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/3896
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming, Peoples R China

Recommended Citation:
Yang, Ying; Zhai, Yanhong; Liu, Tao; Zhang, Fangming; Ji, Yunheng.Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region,PLANTA MEDICA,2011,77(1):87-91
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