Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region
Yang, Ying1,2; Zhai, Yanhong1,2; Liu, Tao3; Zhang, Fangming1,2; Ji, Yunheng1
2011
发表期刊PLANTA MEDICA
ISSN0032-0943
卷号77期号:1页码:87-91
摘要Two varieties of Paris polyphylla Smith (Melanthiaceae), P. polyphylla Smith var. chinensis (Franch.) Hara, and P. polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz., are used as medicinal Paris in China. Their dried rhizomes are the major source of raw material for some medicines. In recent years, medicinal Paris has been found to be adulterated with Valeriana jatamansi Jones (Valerianaceae) due to its high market demand and natural resource deficiency. After the chloroplast psbA-trnH regions of medicinal Paris and V. jatamansi were sequenced and analyzed, it was found that their characteristic sizes were > 1000 and around 250 bp, respectively. Based on length variation, medicinal Paris and the mixed adulterant were detected and distinguished from each other by amplification and electrophoresis. The amount of V. jatamansi that can be identified as an adulterant of medicinal Paris was also investigated. A trace amount (1 : 1000) of the adulterant was detected in the sensitivity tests. The established method has been proven to be sensitive and reliable.
关键词Paris Polyphylla Var. Chinensis Paris Polyphylla Var. Yunnanensis Melanthiaceae Valeriana Jatamansi Valerianaceae Molecular Authentication Psba-trnh
资助信息Natural Science Foundation of China (NSFC)[30670132]; Natural Science Foundation of Yunnan Province (NSFN)[2007C089M]; State Key Laboratory of Phytochemistry and Plant Resources in West China; Germplasm Bank of Wild Species; CAS of the Kunming Institute of Botany
学科领域Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI10.1055/s-0030-1250072
收录类别SCI
语种英语
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy
WOS类目Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号WOS:000285406500018
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3896
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming, Peoples R China
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Yang, Ying,Zhai, Yanhong,Liu, Tao,et al. Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region[J]. PLANTA MEDICA,2011,77(1):87-91.
APA Yang, Ying,Zhai, Yanhong,Liu, Tao,Zhang, Fangming,&Ji, Yunheng.(2011).Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region.PLANTA MEDICA,77(1),87-91.
MLA Yang, Ying,et al."Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region".PLANTA MEDICA 77.1(2011):87-91.
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