Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation

doi:10.1371/journal.pone.0020714

	Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation
	Bai, Xuegui 1; Yang, Liming 3; Tian, Meihua 1; Chen, Jinhui 2; Shi, Jisen 2; Yang, Yongping1 ; Hu, Xiangyang 1
通讯作者	huxiangyang@mail.kib.ac.cn
	2011-06-02
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	6 期号:6 页码:e20714
摘要	The viability of recalcitrant seeds is lost following stress from either drying or freezing. Reactive oxygen species (ROS) resulting from uncontrolled metabolic activity are likely responsible for seed sensitivity to drying. Nitric oxide (NO) and the ascorbate-glutathione cycle can be used for the detoxification of ROS, but their roles in the seed response to desiccation remain poorly understood. Here, we report that desiccation induces rapid accumulation of H2O2, which blocks recalcitrant Antiaris toxicaria seed germination; however, pretreatment with NO increases the activity of antioxidant ascorbate-glutathione pathway enzymes and metabolites, diminishes H2O2 production and assuages the inhibitory effects of desiccation on seed germination. Desiccation increases the protein carbonylation levels and reduces protein S-nitrosylation of these antioxidant enzymes; these effects can be reversed with NO treatment. Antioxidant protein S-nitrosylation levels can be further increased by the application of S-nitrosoglutathione reductase inhibitors, which further enhances NO-induced seed germination rates after desiccation and reduces desiccation-induced H2O2 accumulation. These findings suggest that NO reinforces recalcitrant seed desiccation tolerance by regulating antioxidant enzyme activities to stabilize H2O2 accumulation at an appropriate concentration. During this process, protein carbonylation and S-nitrosylation patterns are used as a specific molecular switch to control antioxidant enzyme activities.
关键词	Hydrogen-peroxide Abscisic-acid Disease Resistance Hydroxyl Radicals Oxidative Stress Stomatal Closure Active Oxygen Cell-walls Zea-mays Arabidopsis
学科领域	Biology
DOI	10.1371/journal.pone.0020714
收录类别	SCI
语种	英语
WOS记录号	WOS:000291310600027
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/3832
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China 2.Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing, Peoples R China 3.Huaiyin Normal Univ, Sch Life Sci, Huaian, Peoples R China
推荐引用方式 GB/T 7714	Bai, Xuegui,Yang, Liming,Tian, Meihua,et al. Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation[J]. PLOS ONE,2011,6(6):e20714.
APA	Bai, Xuegui.,Yang, Liming.,Tian, Meihua.,Chen, Jinhui.,Shi, Jisen.,...&Hu, Xiangyang.(2011).Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation.PLOS ONE,6(6),e20714.
MLA	Bai, Xuegui,et al."Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation".PLOS ONE 6.6(2011):e20714.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
201203160005.pdf（1170KB）			开放获取	--	浏览下载