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Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis
Huang, Weiping1,2; Ye, Jingrun1,2; Zhang, Jingjing1; Lin, Yan1; He, Mingxia1; Huang, Junchao1
2016-07-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume17Pages:236-243
AbstractGlucose induces the green alga Chlorella zofingiensis to accumulate high amounts of astaxanthin and triacylglycerol (TAG). Here we used comparative transcriptome analysis to identify the genes and their expressions for the biosynthesis of the important metabolites. Transcriptome data revealed a great number of gene coding sequences involved in most primary and secondary metabolisms, including full coding sequences of all the genes for astaxanthin and TAG biosynthesis. The biosynthesis of IPP (isopentenyl diphosphate) by the MEP (methylerythritol 4-phosphate) pathway was found to be correlated to astaxanthin biosynthesis. The enhanced astaxanthin synthesis induced by glucose was closely related to the up-regulation of astaxanthin pathway genes coupled with the repression of side pathway genes. Most of the genes involved in fatty acid and TAG biosynthesis consist of more than one copy. Their global up-regulation triggered off the accumulation of fatty acids and TAG in C. zofingiensis. In addition, glucose up-regulated DGAT2 (diacylglycerol acyltransferase 2) but down-regulated PDAT (phospholipid: diacylglycerol acyltransferase), suggesting that the acyl-CoA-dependent pathway attributed to the accumulation of TAG. Astaxanthin and TAG accumulations were found to be coordinated. Our transcriptome datasets can serve as a valuable and informative platform for functional studies on carotenoids, lipids and other important metabolites. (C) 2016 Elsevier B.V. All rights reserved.
KeywordAstaxanthin Chlorella Zofingiensis Fatty Acid Transcriptome
DOI10.1016/j.algal.2016.05.015
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000378863700026
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/26300
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan Province, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Huang, Weiping,Ye, Jingrun,Zhang, Jingjing,et al. Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,17:236-243.
APA Huang, Weiping,Ye, Jingrun,Zhang, Jingjing,Lin, Yan,He, Mingxia,&Huang, Junchao.(2016).Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,17,236-243.
MLA Huang, Weiping,et al."Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 17(2016):236-243.
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