| Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis | |
Huang, Weiping1,2; Ye, Jingrun1,2; Zhang, Jingjing1; Lin, Yan1; He, Mingxia1 ; Huang, Junchao1
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| 2016-07-01 | |
| 发表期刊 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| 卷号 | 17页码:236-243 |
| 摘要 | Glucose induces the green alga Chlorella zofingiensis to accumulate high amounts of astaxanthin and triacylglycerol (TAG). Here we used comparative transcriptome analysis to identify the genes and their expressions for the biosynthesis of the important metabolites. Transcriptome data revealed a great number of gene coding sequences involved in most primary and secondary metabolisms, including full coding sequences of all the genes for astaxanthin and TAG biosynthesis. The biosynthesis of IPP (isopentenyl diphosphate) by the MEP (methylerythritol 4-phosphate) pathway was found to be correlated to astaxanthin biosynthesis. The enhanced astaxanthin synthesis induced by glucose was closely related to the up-regulation of astaxanthin pathway genes coupled with the repression of side pathway genes. Most of the genes involved in fatty acid and TAG biosynthesis consist of more than one copy. Their global up-regulation triggered off the accumulation of fatty acids and TAG in C. zofingiensis. In addition, glucose up-regulated DGAT2 (diacylglycerol acyltransferase 2) but down-regulated PDAT (phospholipid: diacylglycerol acyltransferase), suggesting that the acyl-CoA-dependent pathway attributed to the accumulation of TAG. Astaxanthin and TAG accumulations were found to be coordinated. Our transcriptome datasets can serve as a valuable and informative platform for functional studies on carotenoids, lipids and other important metabolites. (C) 2016 Elsevier B.V. All rights reserved. |
| 关键词 | Astaxanthin Chlorella Zofingiensis Fatty Acid Transcriptome |
| DOI | 10.1016/j.algal.2016.05.015 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378863700026 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/26300 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan Province, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Weiping,Ye, Jingrun,Zhang, Jingjing,et al. Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,17:236-243. |
| APA | Huang, Weiping,Ye, Jingrun,Zhang, Jingjing,Lin, Yan,He, Mingxia,&Huang, Junchao.(2016).Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,17,236-243. |
| MLA | Huang, Weiping,et al."Transcriptome analysis of Chlorella zofingiensis to identify genes and their expressions involved in astaxanthin and triacylglycerol biosynthesis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 17(2016):236-243. |
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