A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum

doi:10.1105/tpc.15.00715

	A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum
	Liu, Yan 1; Luo, Shi-Hong 1; Schmidt, Axel 2; Wang, Guo-Dong 3,4; Sun, Gui-Ling5 ; Grant, Marcus 2; Kuang, Ce 1; Yang, Min-Jie 1; Jing, Shu-Xi 1; Li, Chun-Huan 1; Schneider, Bernd 2; Gershenzon, Jonathan 2; Li, Sheng-Hong1
	2016-03-01
发表期刊	PLANT CELL
卷号	28 期号:3 页码:804-822
摘要	Plant sesterterpenoids, an important class of terpenoids, are widely distributed in various plants, including food crops. However, little is known about their biosynthesis. Here, we cloned and functionally characterized a plant geranylfarnesyl diphosphate synthase (Lc-GFDPS), the enzyme producing the C-25 prenyl diphosphate precursor to all sesterterpenoids, from the glandular trichomes of the woody plant Leucosceptrum canum. GFDPS catalyzed the formation of GFDP after expression in Escherichia coli. Overexpressing GFDPS in Arabidopsis thaliana also gave an extract catalyzing GFDP formation. GFDPS was strongly expressed in glandular trichomes, and its transcript profile was completely in accordance with the sesterterpenoid accumulation pattern. GFDPS is localized to the plastids, and inhibitor studies indicated its use of isoprenyl diphosphate substrates supplied by the 2-C-methyl-D-erythritol 4-phosphate pathway. Application of a jasmonate defense hormone induced GFDPS transcript and sesterterpenoid accumulation, while reducing feeding and growth of the generalist insect Spodoptera exigua, suggesting that these C-25 terpenoids play a defensive role. Phylogenetic analysis suggested that GFDPS probably evolved from plant geranylgeranyl diphosphate synthase under the influence of positive selection. The isolation of GFDPS provides a model for investigating sesterterpenoid formation in other species and a tool for manipulating the formation of this group in plants and other organisms.
DOI	10.1105/tpc.15.00715
收录类别	SCI
语种	英语
WOS记录号	WOS:000375415100018
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/26225
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China 2.Max Planck Inst Chem Ecol, D-07745 Jena, Germany 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yan,Luo, Shi-Hong,Schmidt, Axel,et al. A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum[J]. PLANT CELL,2016,28(3):804-822.
APA	Liu, Yan.,Luo, Shi-Hong.,Schmidt, Axel.,Wang, Guo-Dong.,Sun, Gui-Ling.,...&Li, Sheng-Hong.(2016).A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum.PLANT CELL,28(3),804-822.
MLA	Liu, Yan,et al."A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum".PLANT CELL 28.3(2016):804-822.

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