A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum
Liu, Yan1; Luo, Shi-Hong1; Schmidt, Axel2; Wang, Guo-Dong3,4; Sun, Gui-Ling5; Grant, Marcus2; Kuang, Ce1; Yang, Min-Jie1; Jing, Shu-Xi1; Li, Chun-Huan1; Schneider, Bernd2; Gershenzon, Jonathan2; Li, Sheng-Hong1
2016-03-01
Source PublicationPLANT CELL
Volume28Issue:3Pages:804-822
AbstractPlant sesterterpenoids, an important class of terpenoids, are widely distributed in various plants, including food crops. However, little is known about their biosynthesis. Here, we cloned and functionally characterized a plant geranylfarnesyl diphosphate synthase (Lc-GFDPS), the enzyme producing the C-25 prenyl diphosphate precursor to all sesterterpenoids, from the glandular trichomes of the woody plant Leucosceptrum canum. GFDPS catalyzed the formation of GFDP after expression in Escherichia coli. Overexpressing GFDPS in Arabidopsis thaliana also gave an extract catalyzing GFDP formation. GFDPS was strongly expressed in glandular trichomes, and its transcript profile was completely in accordance with the sesterterpenoid accumulation pattern. GFDPS is localized to the plastids, and inhibitor studies indicated its use of isoprenyl diphosphate substrates supplied by the 2-C-methyl-D-erythritol 4-phosphate pathway. Application of a jasmonate defense hormone induced GFDPS transcript and sesterterpenoid accumulation, while reducing feeding and growth of the generalist insect Spodoptera exigua, suggesting that these C-25 terpenoids play a defensive role. Phylogenetic analysis suggested that GFDPS probably evolved from plant geranylgeranyl diphosphate synthase under the influence of positive selection. The isolation of GFDPS provides a model for investigating sesterterpenoid formation in other species and a tool for manipulating the formation of this group in plants and other organisms.
DOI10.1105/tpc.15.00715
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS IDWOS:000375415100018
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/26225
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
2.Max Planck Inst Chem Ecol, D-07745 Jena, Germany
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yan,Luo, Shi-Hong,Schmidt, Axel,et al. A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum[J]. PLANT CELL,2016,28(3):804-822.
APA Liu, Yan.,Luo, Shi-Hong.,Schmidt, Axel.,Wang, Guo-Dong.,Sun, Gui-Ling.,...&Li, Sheng-Hong.(2016).A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum.PLANT CELL,28(3),804-822.
MLA Liu, Yan,et al."A Geranylfarnesyl Diphosphate Synthase Provides the Precursor for Sesterterpenoid (C-25) Formation in the Glandular Trichomes of the Mint Species Leucosceptrum canum".PLANT CELL 28.3(2016):804-822.
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