Strong genetic differentiation of Primula sikkimensis in the East Himalaya-Hengduan Mountains

doi:10.1007/s10528-007-9131-9

	Strong genetic differentiation of Primula sikkimensis in the East Himalaya-Hengduan Mountains
	Wang, Feng-Ying 1,2; Ge, Xue-Jun 3; Gong, Xun4 ; Hu, Chi-Ming 1; Hao, Gang
通讯作者	haogang07@gmail.com
	2008-02-01
发表期刊	BIOCHEMICAL GENETICS
ISSN	0006-2928
卷号	46 期号:1-2 页码:75-87
摘要	The East Himalaya-Hengduan Mountains region is the center of diversity of the genus Primula, and P. sikkimensis is one of the most common members of the genus in the region. In this study, the genetic diversity and structure of P. sikkimensis populations in China were assessed using inter-simple sequence repeat (ISSR) and chloroplast microsatellite markers. The 254 individuals analyzed represented 13 populations. High levels of genetic diversity were revealed by ISSR markers. At the species level, the expected heterozygosity and Shannon's index were 0.4032 and 0.5576, respectively. AMOVA analysis showed that 50.3% of the total genetic diversity was partitioned among populations. Three pairs of chloroplast microsatellite primers tested yielded a total of 12 size variants and 15 chloroplast haplotypes. Strong cpDNA genetic differentiation (G(ST) = 0.697) and evidence for phylogeographic structure were detected (N-ST = 0.788, significantly higher than G(ST)). Estimated rates of pollen-mediated gene flow are approximately 27% greater than estimated rates of seed-mediated gene flow in P. sikkimensis. Both seed and pollen dispersal, however, are limited, and gene flow among populations appears to be hindered by the patchiness of the species' habitats and their geographic isolation. These features may have played important roles in shaping the genetic structure of P. sikkimensis. A minimum-spanning tree of chloroplast DNA haplotypes was constructed, and possible glacial refugia of P. sikkimensis were identified.
关键词	Chloroplast Microsatellite East Himalaya-hengduan Mountains Issr Primula Sikkimensis
学科领域	Biochemistry & Molecular Biology ; Genetics & Heredity
DOI	10.1007/s10528-007-9131-9
收录类别	SCI
语种	英语
WOS记录号	WOS:000252766900008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2523
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Peoples R China 2.Shanghai Bot Garden, Shanghai 200231, Peoples R China 3.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Wang, Feng-Ying,Ge, Xue-Jun,Gong, Xun,et al. Strong genetic differentiation of Primula sikkimensis in the East Himalaya-Hengduan Mountains[J]. BIOCHEMICAL GENETICS,2008,46(1-2):75-87.
APA	Wang, Feng-Ying,Ge, Xue-Jun,Gong, Xun,Hu, Chi-Ming,&Hao, Gang.(2008).Strong genetic differentiation of Primula sikkimensis in the East Himalaya-Hengduan Mountains.BIOCHEMICAL GENETICS,46(1-2),75-87.
MLA	Wang, Feng-Ying,et al."Strong genetic differentiation of Primula sikkimensis in the East Himalaya-Hengduan Mountains".BIOCHEMICAL GENETICS 46.1-2(2008):75-87.

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