Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)

doi:10.1007/s10592-007-9391-2

	Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
	Hung, Cheng-Yu 1; Wang, Kuo-Hsiung 1; Huang, Chi-Chun 1; Gong, Xun2 ; Ge, Xue-Jun 3; Chiang, Tzen-Yuh 1
通讯作者	tychiang@mail.ncku.edu.tw
	2008-06-01
发表期刊	CONSERVATION GENETICS
ISSN	1566-0621
卷号	9 期号:3 页码:779-781
摘要	We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H(E)) and observed (H(O)) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.
关键词	Camellia Sinensis Heterozygosity Microsatellite Fingerprinting Pima Rapd-pcr Enrichment
学科领域	Biodiversity Conservation ; Genetics & Heredity
DOI	10.1007/s10592-007-9391-2
收录类别	SCI
语种	英语
WOS记录号	WOS:000255191000038
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2515
专题	资源植物与生物技术所级重点实验室
作者单位	1.Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 3.Sun Yat Sen Univ, State Key Lab Biocontrol Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,et al. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)[J]. CONSERVATION GENETICS,2008,9(3):779-781.
APA	Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,Gong, Xun,Ge, Xue-Jun,&Chiang, Tzen-Yuh.(2008).Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA).CONSERVATION GENETICS,9(3),779-781.
MLA	Hung, Cheng-Yu,et al."Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)".CONSERVATION GENETICS 9.3(2008):779-781.

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