Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
Hung, Cheng-Yu1; Wang, Kuo-Hsiung1; Huang, Chi-Chun1; Gong, Xun2; Ge, Xue-Jun3; Chiang, Tzen-Yuh1
2008-06-01
发表期刊CONSERVATION GENETICS
ISSN1566-0621
卷号9期号:3页码:779-781
摘要We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H(E)) and observed (H(O)) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.
关键词Camellia Sinensis Heterozygosity Microsatellite Fingerprinting Pima Rapd-pcr Enrichment
学科领域Biodiversity Conservation ; Genetics & Heredity
DOI10.1007/s10592-007-9391-2
收录类别SCI
语种英语
WOS研究方向Biodiversity & Conservation ; Genetics & Heredity
WOS类目Biodiversity Conservation ; Genetics & Heredity
WOS记录号WOS:000255191000038
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2515
专题资源植物与生物技术所级重点实验室
作者单位1.Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
3.Sun Yat Sen Univ, State Key Lab Biocontrol Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
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Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,et al. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)[J]. CONSERVATION GENETICS,2008,9(3):779-781.
APA Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,Gong, Xun,Ge, Xue-Jun,&Chiang, Tzen-Yuh.(2008).Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA).CONSERVATION GENETICS,9(3),779-781.
MLA Hung, Cheng-Yu,et al."Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)".CONSERVATION GENETICS 9.3(2008):779-781.
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