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Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
Hung, Cheng-Yu1; Wang, Kuo-Hsiung1; Huang, Chi-Chun1; Gong, Xun2; Ge, Xue-Jun3; Chiang, Tzen-Yuh1
Corresponding Authortychiang@mail.ncku.edu.tw
2008-06-01
Source PublicationCONSERVATION GENETICS
ISSN1566-0621
Volume9Issue:3Pages:779-781
AbstractWe report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H(E)) and observed (H(O)) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.
KeywordCamellia Sinensis Heterozygosity Microsatellite Fingerprinting Pima Rapd-pcr Enrichment
Subject AreaBiodiversity Conservation ; Genetics & Heredity
DOI10.1007/s10592-007-9391-2
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000255191000038
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/2515
Collection资源植物与生物技术所级重点实验室
Affiliation1.Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
3.Sun Yat Sen Univ, State Key Lab Biocontrol Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,et al. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)[J]. CONSERVATION GENETICS,2008,9(3):779-781.
APA Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,Gong, Xun,Ge, Xue-Jun,&Chiang, Tzen-Yuh.(2008).Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA).CONSERVATION GENETICS,9(3),779-781.
MLA Hung, Cheng-Yu,et al."Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)".CONSERVATION GENETICS 9.3(2008):779-781.
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