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题名: Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
作者: Hung, Cheng-Yu1; Wang, Kuo-Hsiung1; Huang, Chi-Chun1; null(龚洵)2; Ge, Xue-Jun3; Chiang, Tzen-Yuh1
刊名: CONSERVATION GENETICS
关键词: Camellia sinensis ; heterozygosity ; microsatellite fingerprinting ; PIMA ; RAPD-PCR enrichment
英文摘要: We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H(E)) and observed (H(O)) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.
出版日期: 2008-06-01
卷号: 9, 期号:3, 页码:779-781
语种: 英语
收录类别: SCI
学科分类: Biodiversity Conservation; Genetics & Heredity
ISSN号: 1566-0621
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2515
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
3.Sun Yat Sen Univ, State Key Lab Biocontrol Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Hung, CY; Wang, KH; Huang, CC; Gong, X; Ge, XJ; Chiang, TY.Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA),CONSERVATION GENETICS,2008,9(3):779-781
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