Structural Characterization and Immunostirnulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

doi:10.1021/acs.jafc.5b03306

	Structural Characterization and Immunostirnulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta
	Yuan,Qingxia; Zhao,Longyan; Cha,Qianqian; Sun,Yi; Ye,Hong; Zeng,Xiaoxiong
	2015-09-16
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号	63 期号:36 页码:7986-7994
摘要	Sinonovacula constricta has been widely used as a health food and medicine in China, Japan, and Korea. In the present study, a water-soluble polysaccharide fraction (SCP-1) was prepared from S. constricta by enzyme-assisted extraction and purification of chromatography with DEAE-52 cellulose anion-exchange column and Sephadex G-100 size exclusion column. On the basis of the analytical results of high-performance liquid chromatography, Fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, SCP-1 was found to have an average molecular weight of 15.63 kDa and a linear backbone of (1 -> 4)-linked alpha-D-Glcp residue with one branch, alpha-D-Glcp, attached to the main chain by a (1 -> 6) glycosidic bond at every five alpha-D-Glcp units. Furthermore, it was found that SCP-1 could significantly increase the viability of macrophages, enhance the capability of macrophage phagocytosis, increase the activity of acid phosphatase, and promote the production of nitric oxide, mouse tumor necrosis factor (TNF)-alpha, mouse interferon (IFN)-gamma, and mouse interleuldn (IL)-1 beta. The results suggest that SCP-1 possesses potent immunomodulating effect and may be explored as a potential biological response modifier.
关键词	Sinonovacula Constricta Polysaccharide Structure Immunomodulatory Activity
DOI	10.1021/acs.jafc.5b03306
收录类别	SCI
语种	英语
WOS记录号	WOS:000361502000012
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/25141
专题	植物化学与西部植物资源持续利用国家重点实验室
推荐引用方式 GB/T 7714	Yuan,Qingxia,Zhao,Longyan,Cha,Qianqian,et al. Structural Characterization and Immunostirnulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015,63(36):7986-7994.
APA	Yuan,Qingxia,Zhao,Longyan,Cha,Qianqian,Sun,Yi,Ye,Hong,&Zeng,Xiaoxiong.(2015).Structural Characterization and Immunostirnulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,63(36),7986-7994.
MLA	Yuan,Qingxia,et al."Structural Characterization and Immunostirnulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63.36(2015):7986-7994.

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