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题名: Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)
作者: Yang, Yang1; Li, Yong2; Li, Lin-Feng2; Ge, Xue-Jun2; null(龚洵)1
刊名: MOLECULAR ECOLOGY RESOURCES
关键词: Cycas debaoensis ; dbEST ; FIASCO ; microsatellite markers ; polymorphism
英文摘要: Cycas debaoensis is one of the critically endangered cycad species endemic to China. In this study, we described the development of six microsatellite markers from the genome of C. debaoensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO) and two microsatellite markers derived from the database of expressed sequence tags (dbEST). Polymorphism of each locus was assessed in 60 adult individuals of the cycad. The average allele number of the microsatellites was 2.6 per locus, ranging from two to five. The observed and expected heterozygosities varied from 0.0833 to 0.7333 and from 0.0805 to 0.7188, respectively. Despite its rarity, only one locus (Y177) deviated from Hardy-Weinberg equilibrium due to the excessive homozygosity. The marker transferability of the eight primer pairs was tested on other four congeneric species that also occur in China.
出版日期: 2008-07-01
卷号: 8, 期号:4, 页码:913-915
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Ecology; Evolutionary Biology
ISSN号: 1471-8278
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2483
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Yang, Y; Li, Y; Li, LF; Ge, XJ; Gong, X.Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae),MOLECULAR ECOLOGY RESOURCES,2008,8(4):913-915
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