Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)
Yang, Yang1; Li, Yong2; Li, Lin-Feng2; Ge, Xue-Jun2; Gong, Xun1
2008-07-01
发表期刊MOLECULAR ECOLOGY RESOURCES
ISSN1471-8278
卷号8期号:4页码:913-915
摘要Cycas debaoensis is one of the critically endangered cycad species endemic to China. In this study, we described the development of six microsatellite markers from the genome of C. debaoensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO) and two microsatellite markers derived from the database of expressed sequence tags (dbEST). Polymorphism of each locus was assessed in 60 adult individuals of the cycad. The average allele number of the microsatellites was 2.6 per locus, ranging from two to five. The observed and expected heterozygosities varied from 0.0833 to 0.7333 and from 0.0805 to 0.7188, respectively. Despite its rarity, only one locus (Y177) deviated from Hardy-Weinberg equilibrium due to the excessive homozygosity. The marker transferability of the eight primer pairs was tested on other four congeneric species that also occur in China.
关键词Cycas Debaoensis Dbest Fiasco Microsatellite Markers Polymorphism
学科领域Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1755-0998.2008.02114.x
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号WOS:000257511600050
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2483
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
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Yang, Yang,Li, Yong,Li, Lin-Feng,et al. Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)[J]. MOLECULAR ECOLOGY RESOURCES,2008,8(4):913-915.
APA Yang, Yang,Li, Yong,Li, Lin-Feng,Ge, Xue-Jun,&Gong, Xun.(2008).Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae).MOLECULAR ECOLOGY RESOURCES,8(4),913-915.
MLA Yang, Yang,et al."Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)".MOLECULAR ECOLOGY RESOURCES 8.4(2008):913-915.
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