ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ.
Nakano, Masaru1; Takagi, Hiroki1; Sugawara, Shintaro1; Saito, Tomoka1; Watanabe, Yusuke1,2; Lu Yuanxue3; Guan Kaiyun3; Godo, Toshinari4
2009-12-01
发表期刊PROPAGATION OF ORNAMENTAL PLANTS
ISSN1311-9109
卷号9期号:4页码:216-222
摘要Chirita flavimaculata, C. eburnea, and C. speciosa, which belong to Gesneriaceae, have high ornamental values such as beautiful foliages and showy flowers. An efficient plant regeneration system via adventitious shoot formation was established for micropropagation of these species. For all species, adventitious shoots elongating over 5 mm in length were efficiently regenerated from leaf explants on half-strength MS medium containing 0.1 mg l(-1) of both NAA and BA (8.1-15.6 shoots per explant). Histological observation indicated that adventitious shoot regeneration on a medium containing 0.1 mg l(-1) of both NAA and BA occurred directly from leaf epidermal cells without callus formation. Plantlets were obtained by rooting the shoots on half-strength MS medium containing 0.1 mg l(-1) IBA. No variations in the ploidy level were observed in the regenerated plants of three Chirita species as indicated by flow cytometry analysis.
关键词Chirita Flow Cytometry Analysis Histological Observation Plant Regeneration Ploidy Stability Rooting
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2435
专题资源植物与生物技术所级重点实验室
作者单位1.Niigata Univ, Fac Agr, Niigata 9502181, Japan
2.Hort Res Ctr, Niigata Agr Res Inst, Seiro 9570111, Japan
3.Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Yunnan, Peoples R China
4.Bot Gardens Toyama, Toyama 9392713, Japan
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Nakano, Masaru,Takagi, Hiroki,Sugawara, Shintaro,et al. ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ.[J]. PROPAGATION OF ORNAMENTAL PLANTS,2009,9(4):216-222.
APA Nakano, Masaru.,Takagi, Hiroki.,Sugawara, Shintaro.,Saito, Tomoka.,Watanabe, Yusuke.,...&Godo, Toshinari.(2009).ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ..PROPAGATION OF ORNAMENTAL PLANTS,9(4),216-222.
MLA Nakano, Masaru,et al."ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ.".PROPAGATION OF ORNAMENTAL PLANTS 9.4(2009):216-222.
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