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题名: ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ.
作者: Nakano, Masaru1; Takagi, Hiroki1; Sugawara, Shintaro1; Saito, Tomoka1; Watanabe, Yusuke1, 2; Lu Yuanxue3; Guan Kaiyun3; Godo, Toshinari4
刊名: PROPAGATION OF ORNAMENTAL PLANTS
关键词: Chirita ; flow cytometry analysis ; histological observation ; plant regeneration ; ploidy stability ; rooting
英文摘要: Chirita flavimaculata, C. eburnea, and C. speciosa, which belong to Gesneriaceae, have high ornamental values such as beautiful foliages and showy flowers. An efficient plant regeneration system via adventitious shoot formation was established for micropropagation of these species. For all species, adventitious shoots elongating over 5 mm in length were efficiently regenerated from leaf explants on half-strength MS medium containing 0.1 mg l(-1) of both NAA and BA (8.1-15.6 shoots per explant). Histological observation indicated that adventitious shoot regeneration on a medium containing 0.1 mg l(-1) of both NAA and BA occurred directly from leaf epidermal cells without callus formation. Plantlets were obtained by rooting the shoots on half-strength MS medium containing 0.1 mg l(-1) IBA. No variations in the ploidy level were observed in the regenerated plants of three Chirita species as indicated by flow cytometry analysis.
出版日期: 2009-12-01
卷号: 9, 期号:4, 页码:216-222
语种: 英语
收录类别: SCI
学科分类: Horticulture
ISSN号: 1311-9109
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2435
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Niigata Univ, Fac Agr, Niigata 9502181, Japan
2.Hort Res Ctr, Niigata Agr Res Inst, Seiro 9570111, Japan
3.Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Yunnan, Peoples R China
4.Bot Gardens Toyama, Toyama 9392713, Japan

Recommended Citation:
Nakano, M; Takagi, H; Sugawara, S; Saito, T; Watanabe, Y; Lu, YX; Guan, KY; Godo, T.ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ.,PROPAGATION OF ORNAMENTAL PLANTS,2009,9(4):216-222
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