In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)
Long, Bo1,2; Niemiera, Alex X.3; Cheng, Zhi-ying1; Long, Chun-lin1,4
2010-05-01
发表期刊PLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
卷号101期号:2页码:151-162
摘要The effects of seed maturity, media type, carbon source, and organic nutrient additives on seed germination, protocorm development, and plant growth of Paphiopedilum villosum var. densissimum Z. J. Liu et S. C. Chen were investigated. Micropropagation frequency was enhanced through the use of 200-day-old seed, Knudson C (KC) medium, and the presence of both glucose and coconut milk in the medium. The effects of various plant growth regulators on the frequency of shoot organogenesis in four Paphiopedilum species were also investigated. Explants of P. villosum var. densissimum and P. insigne (Lindl.) Stein incubated in the presence of 5 mg l(-1) 6-benzyladenine (BA) with 0.5 mg l(-1) alpha-naphthalene acetic acid (NAA) and 0.2 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, showed a twofold increase in the frequency of shoot organogenesis. For explants of P. bellatulum (Rchb. f.) Stein and P. armeniacum S. C. Chen et F. Y. Liu, the combination of 5.5 mg l(-1) BA with 0.5 mg l(-1) NAA and 4 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, resulted in the highest frequencies of shoot organogenesis.
关键词Micropropagation Orchid Seed Germination Shoot Proliferation
资助信息The authors thank Chunyan Han, Guangwan Hu, and Yuan Liu for their kind help. This work was supported by the Natural Science Foundation of Yunnan (2005C0053 M), the Ministry of Science and Technology of China (2005DK21006) and the Ministry of Education of China (B08044 & MUC-985-3-3).
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2431
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Virginia Polytech & State Univ, Dept Hort, Blacksburg, VA 24061 USA
4.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
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Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,et al. In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,101(2):151-162.
APA Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,&Long, Chun-lin.(2010).In vitro propagation of four threatened Paphiopedilum species (Orchidaceae).PLANT CELL TISSUE AND ORGAN CULTURE,101(2),151-162.
MLA Long, Bo,et al."In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)".PLANT CELL TISSUE AND ORGAN CULTURE 101.2(2010):151-162.
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