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In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)
Long, Bo1,2; Niemiera, Alex X.3; Cheng, Zhi-ying1; Long, Chun-lin1,4
Corresponding Authorlongbo@mail.kib.ac.cn ; niemiera@vt.edu ; long@mail.kib.ac.cn
2010-05-01
Source PublicationPLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
Volume101Issue:2Pages:151-162
AbstractThe effects of seed maturity, media type, carbon source, and organic nutrient additives on seed germination, protocorm development, and plant growth of Paphiopedilum villosum var. densissimum Z. J. Liu et S. C. Chen were investigated. Micropropagation frequency was enhanced through the use of 200-day-old seed, Knudson C (KC) medium, and the presence of both glucose and coconut milk in the medium. The effects of various plant growth regulators on the frequency of shoot organogenesis in four Paphiopedilum species were also investigated. Explants of P. villosum var. densissimum and P. insigne (Lindl.) Stein incubated in the presence of 5 mg l(-1) 6-benzyladenine (BA) with 0.5 mg l(-1) alpha-naphthalene acetic acid (NAA) and 0.2 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, showed a twofold increase in the frequency of shoot organogenesis. For explants of P. bellatulum (Rchb. f.) Stein and P. armeniacum S. C. Chen et F. Y. Liu, the combination of 5.5 mg l(-1) BA with 0.5 mg l(-1) NAA and 4 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, resulted in the highest frequencies of shoot organogenesis.
KeywordMicropropagation Orchid Seed Germination Shoot Proliferation
Subject AreaBiotechnology & Applied Microbiology ; Plant Sciences
DOI10.1007/s11240-010-9672-1
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000277204500005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/2431
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Virginia Polytech & State Univ, Dept Hort, Blacksburg, VA 24061 USA
4.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,et al. In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,101(2):151-162.
APA Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,&Long, Chun-lin.(2010).In vitro propagation of four threatened Paphiopedilum species (Orchidaceae).PLANT CELL TISSUE AND ORGAN CULTURE,101(2),151-162.
MLA Long, Bo,et al."In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)".PLANT CELL TISSUE AND ORGAN CULTURE 101.2(2010):151-162.
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