In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)

doi:10.1007/s11240-010-9672-1

	In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)
	Long, Bo 1,2; Niemiera, Alex X.3; Cheng, Zhi-ying 1; Long, Chun-lin 1,4
通讯作者	longbo@mail.kib.ac.cn ; niemiera@vt.edu ; long@mail.kib.ac.cn
	2010-05-01
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
卷号	101 期号:2 页码:151-162
摘要	The effects of seed maturity, media type, carbon source, and organic nutrient additives on seed germination, protocorm development, and plant growth of Paphiopedilum villosum var. densissimum Z. J. Liu et S. C. Chen were investigated. Micropropagation frequency was enhanced through the use of 200-day-old seed, Knudson C (KC) medium, and the presence of both glucose and coconut milk in the medium. The effects of various plant growth regulators on the frequency of shoot organogenesis in four Paphiopedilum species were also investigated. Explants of P. villosum var. densissimum and P. insigne (Lindl.) Stein incubated in the presence of 5 mg l(-1) 6-benzyladenine (BA) with 0.5 mg l(-1) alpha-naphthalene acetic acid (NAA) and 0.2 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, showed a twofold increase in the frequency of shoot organogenesis. For explants of P. bellatulum (Rchb. f.) Stein and P. armeniacum S. C. Chen et F. Y. Liu, the combination of 5.5 mg l(-1) BA with 0.5 mg l(-1) NAA and 4 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, resulted in the highest frequencies of shoot organogenesis.
关键词	Micropropagation Orchid Seed Germination Shoot Proliferation
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11240-010-9672-1
收录类别	SCI
语种	英语
WOS记录号	WOS:000277204500005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2431
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Virginia Polytech & State Univ, Dept Hort, Blacksburg, VA 24061 USA 4.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,et al. In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,101(2):151-162.
APA	Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,&Long, Chun-lin.(2010).In vitro propagation of four threatened Paphiopedilum species (Orchidaceae).PLANT CELL TISSUE AND ORGAN CULTURE,101(2),151-162.
MLA	Long, Bo,et al."In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)".PLANT CELL TISSUE AND ORGAN CULTURE 101.2(2010):151-162.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
201112050004.pdf（455KB）			开放获取	--	浏览请求全文