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题名: In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)
作者: Long, Bo1, 2; Niemiera, Alex X.3; Cheng, Zhi-ying1; Long, Chun-lin1, 4
刊名: PLANT CELL TISSUE AND ORGAN CULTURE
关键词: Micropropagation ; Orchid ; Seed germination ; Shoot proliferation
英文摘要: The effects of seed maturity, media type, carbon source, and organic nutrient additives on seed germination, protocorm development, and plant growth of Paphiopedilum villosum var. densissimum Z. J. Liu et S. C. Chen were investigated. Micropropagation frequency was enhanced through the use of 200-day-old seed, Knudson C (KC) medium, and the presence of both glucose and coconut milk in the medium. The effects of various plant growth regulators on the frequency of shoot organogenesis in four Paphiopedilum species were also investigated. Explants of P. villosum var. densissimum and P. insigne (Lindl.) Stein incubated in the presence of 5 mg l(-1) 6-benzyladenine (BA) with 0.5 mg l(-1) alpha-naphthalene acetic acid (NAA) and 0.2 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, showed a twofold increase in the frequency of shoot organogenesis. For explants of P. bellatulum (Rchb. f.) Stein and P. armeniacum S. C. Chen et F. Y. Liu, the combination of 5.5 mg l(-1) BA with 0.5 mg l(-1) NAA and 4 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, resulted in the highest frequencies of shoot organogenesis.
出版日期: 2010-05-01
卷号: 101, 期号:2, 页码:151-162
DOI标识: 10.1007/s11240-010-9672-1
语种: 英语
ISSN号: 0167-6857
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2431
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Virginia Polytech & State Univ, Dept Hort, Blacksburg, VA 24061 USA
4.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China

Recommended Citation:
Long, B; Niemiera, AX; Cheng, ZY; Long, CL.In vitro propagation of four threatened Paphiopedilum species (Orchidaceae),PLANT CELL TISSUE AND ORGAN CULTURE,2010,101(2):151-162
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