Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds

doi:10.1007/s11240-005-9001-2

	Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds
	Yan, N ; Hu, H; Huang, JL; Xu, K ; Wang, H; Zhou, ZK
通讯作者	huhong@mail.kib.ac.cn
	2006
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
卷号	84 期号:1 页码:113-117
摘要	Cypripedium flavum, known as the rare lady's slipper orchid, is one of the endemics with a yellow flower in China. Due to its conservation and commercial requirement, establishment of an efficient method for micropropogation is urgently needed. Multiple shoots were obtained by placing seedlings from seeds of C. flavum on Harvais media supplemented with two cytokinins (BAP or KIN) used alone or in addition to different concentration of potato homogenate. The effect of BAP was better than that of KIN on shoot multiplication. The Havais media supplemented with BAP (2.22 mu M) and potato homogenate (20 g l(-1)) was the most effective, providing high shoot multiplication frequencies (95%) associated with a high number of shoots per explant (2.55 shoots/plant). For root formation, high rooting and survival were achieved using 1/2 Harvais media supplemented with 0.6 g l(-1) activated charcoals. High-level activated charcoal increased the number and the length of roots because the activated charcoal could absorb BAP in the media. This study demonstrated that C. flavum could be micropropagated by using multiple shoots of seedlings derived from mature seeds.
关键词	Cypripedium Flavum Conservation Induction Of Multiple Shoots Micropropagation Root Formation
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11240-005-9001-2
收录类别	SCI
语种	英语
WOS记录号	WOS:000236371200011
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2429
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yan, N,Hu, H,Huang, JL,et al. Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2006,84(1):113-117.
APA	Yan, N,Hu, H,Huang, JL,Xu, K,Wang, H,&Zhou, ZK.(2006).Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds.PLANT CELL TISSUE AND ORGAN CULTURE,84(1),113-117.
MLA	Yan, N,et al."Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds".PLANT CELL TISSUE AND ORGAN CULTURE 84.1(2006):113-117.

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