KIB OpenIR  > 资源植物与生物技术所级重点实验室
Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds
Yan, N; Hu, H; Huang, JL; Xu, K; Wang, H; Zhou, ZK
Corresponding Authorhuhong@mail.kib.ac.cn
2006
Source PublicationPLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
Volume84Issue:1Pages:113-117
AbstractCypripedium flavum, known as the rare lady's slipper orchid, is one of the endemics with a yellow flower in China. Due to its conservation and commercial requirement, establishment of an efficient method for micropropogation is urgently needed. Multiple shoots were obtained by placing seedlings from seeds of C. flavum on Harvais media supplemented with two cytokinins (BAP or KIN) used alone or in addition to different concentration of potato homogenate. The effect of BAP was better than that of KIN on shoot multiplication. The Havais media supplemented with BAP (2.22 mu M) and potato homogenate (20 g l(-1)) was the most effective, providing high shoot multiplication frequencies (95%) associated with a high number of shoots per explant (2.55 shoots/plant). For root formation, high rooting and survival were achieved using 1/2 Harvais media supplemented with 0.6 g l(-1) activated charcoals. High-level activated charcoal increased the number and the length of roots because the activated charcoal could absorb BAP in the media. This study demonstrated that C. flavum could be micropropagated by using multiple shoots of seedlings derived from mature seeds.
KeywordCypripedium Flavum Conservation Induction Of Multiple Shoots Micropropagation Root Formation
Subject AreaBiotechnology & Applied Microbiology ; Plant Sciences
DOI10.1007/s11240-005-9001-2
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000236371200011
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/2429
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yan, N,Hu, H,Huang, JL,et al. Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2006,84(1):113-117.
APA Yan, N,Hu, H,Huang, JL,Xu, K,Wang, H,&Zhou, ZK.(2006).Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds.PLANT CELL TISSUE AND ORGAN CULTURE,84(1),113-117.
MLA Yan, N,et al."Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds".PLANT CELL TISSUE AND ORGAN CULTURE 84.1(2006):113-117.
Files in This Item: Download All
File Name/Size DocType Version Access License
201112050005.pdf(261KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yan, N]'s Articles
[Hu, H]'s Articles
[Huang, JL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yan, N]'s Articles
[Hu, H]'s Articles
[Huang, JL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yan, N]'s Articles
[Hu, H]'s Articles
[Huang, JL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 201112050005.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.