Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds | |
Yan, N; Hu, H![]() ![]() ![]() ![]() | |
通讯作者 | huhong@mail.kib.ac.cn |
2006 | |
发表期刊 | PLANT CELL TISSUE AND ORGAN CULTURE
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ISSN | 0167-6857 |
卷号 | 84期号:1页码:113-117 |
摘要 | Cypripedium flavum, known as the rare lady's slipper orchid, is one of the endemics with a yellow flower in China. Due to its conservation and commercial requirement, establishment of an efficient method for micropropogation is urgently needed. Multiple shoots were obtained by placing seedlings from seeds of C. flavum on Harvais media supplemented with two cytokinins (BAP or KIN) used alone or in addition to different concentration of potato homogenate. The effect of BAP was better than that of KIN on shoot multiplication. The Havais media supplemented with BAP (2.22 mu M) and potato homogenate (20 g l(-1)) was the most effective, providing high shoot multiplication frequencies (95%) associated with a high number of shoots per explant (2.55 shoots/plant). For root formation, high rooting and survival were achieved using 1/2 Harvais media supplemented with 0.6 g l(-1) activated charcoals. High-level activated charcoal increased the number and the length of roots because the activated charcoal could absorb BAP in the media. This study demonstrated that C. flavum could be micropropagated by using multiple shoots of seedlings derived from mature seeds. |
关键词 | Cypripedium Flavum Conservation Induction Of Multiple Shoots Micropropagation Root Formation |
学科领域 | Biotechnology & Applied Microbiology ; Plant Sciences |
DOI | 10.1007/s11240-005-9001-2 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000236371200011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/2429 |
专题 | 资源植物与生物技术所级重点实验室 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Yan, N,Hu, H,Huang, JL,et al. Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2006,84(1):113-117. |
APA | Yan, N,Hu, H,Huang, JL,Xu, K,Wang, H,&Zhou, ZK.(2006).Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds.PLANT CELL TISSUE AND ORGAN CULTURE,84(1),113-117. |
MLA | Yan, N,et al."Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds".PLANT CELL TISSUE AND ORGAN CULTURE 84.1(2006):113-117. |
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