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题名: Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds
作者: Yan, N; null(胡虹); null(黄锦岭); Xu, K; null(王红); null(周浙昆)
刊名: PLANT CELL TISSUE AND ORGAN CULTURE
关键词: Cypripedium flavum ; conservation ; induction of multiple shoots ; micropropagation ; root formation
英文摘要: Cypripedium flavum, known as the rare lady's slipper orchid, is one of the endemics with a yellow flower in China. Due to its conservation and commercial requirement, establishment of an efficient method for micropropogation is urgently needed. Multiple shoots were obtained by placing seedlings from seeds of C. flavum on Harvais media supplemented with two cytokinins (BAP or KIN) used alone or in addition to different concentration of potato homogenate. The effect of BAP was better than that of KIN on shoot multiplication. The Havais media supplemented with BAP (2.22 mu M) and potato homogenate (20 g l(-1)) was the most effective, providing high shoot multiplication frequencies (95%) associated with a high number of shoots per explant (2.55 shoots/plant). For root formation, high rooting and survival were achieved using 1/2 Harvais media supplemented with 0.6 g l(-1) activated charcoals. High-level activated charcoal increased the number and the length of roots because the activated charcoal could absorb BAP in the media. This study demonstrated that C. flavum could be micropropagated by using multiple shoots of seedlings derived from mature seeds.
出版日期: 2006
卷号: 84, 期号:1, 页码:113-117
DOI标识: 10.1007/s11240-005-9001-2
语种: 英语
ISSN号: 0167-6857
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2429
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Yan, N; Hu, H; Huang, JL; Xu, K; Wang, H; Zhou, ZK.Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds,PLANT CELL TISSUE AND ORGAN CULTURE,2006,84(1):113-117
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