Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay
Li, Jiaru1; Yang, Dingyu1; Yu, Kun1; He, Ji1; Zhang, Yingjun2
2010-11-01
Source PublicationPLANTA MEDICA
Volume76Issue:16Pages:1915-1920
AbstractMany medicinal plants contain diosgenin, which has a significant medicinal value. However, there is currently no effective and rapid analytical method to determine the diosgenin content of plants or products. In the present work we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of diosgenin in herbal medicines. Diosgenin was conjugated with bovine serum albumin (BSA) for immunization. A polyclonal antibody developed in rabbits against a diosgenin-BSA conjugate was shown to be specific for diosgenin. The developed ELISA assay was highly sensitive, specific, and easy to perform. In addition, it gave more precise results with less variation than other methods that have been used in the past, including gravimetric and spectrophotometric assays, and correlated well with high-performance liquid chromatography. The diosgenin content determined by ELISA varied widely, with the highest and lowest values in rhizomes or tubers of Paris polyphylla and Dioscorea opposita Thunb. "Jiao-ban Yam", respectively, differing by more than 9000-fold. These results suggest that the ELISA method can be used as a rapid, simple, sensitive, and accurate tool for quantitative analysis of samples containing diosgenin, and may provide an important criterion for quality evaluation and a valuable tool for quality control of diosgenin-containing medicinal plants.
KeywordDiosgenin Elisa Hplc Medicinal Plants
DOI10.1055/s-0030-1250054
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000284144400026
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/23913
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Wuhan Univ, Coll Life Sci, Key Lab Minist Educ Plant Dev Biol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Peoples R China
Recommended Citation
GB/T 7714
Li, Jiaru,Yang, Dingyu,Yu, Kun,et al. Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay[J]. PLANTA MEDICA,2010,76(16):1915-1920.
APA Li, Jiaru,Yang, Dingyu,Yu, Kun,He, Ji,&Zhang, Yingjun.(2010).Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay.PLANTA MEDICA,76(16),1915-1920.
MLA Li, Jiaru,et al."Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay".PLANTA MEDICA 76.16(2010):1915-1920.
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