Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay
Li, Jiaru1; Yang, Dingyu1; Yu, Kun1; He, Ji1; Zhang, Yingjun2
2010-11-01
发表期刊PLANTA MEDICA
卷号76期号:16页码:1915-1920
摘要Many medicinal plants contain diosgenin, which has a significant medicinal value. However, there is currently no effective and rapid analytical method to determine the diosgenin content of plants or products. In the present work we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of diosgenin in herbal medicines. Diosgenin was conjugated with bovine serum albumin (BSA) for immunization. A polyclonal antibody developed in rabbits against a diosgenin-BSA conjugate was shown to be specific for diosgenin. The developed ELISA assay was highly sensitive, specific, and easy to perform. In addition, it gave more precise results with less variation than other methods that have been used in the past, including gravimetric and spectrophotometric assays, and correlated well with high-performance liquid chromatography. The diosgenin content determined by ELISA varied widely, with the highest and lowest values in rhizomes or tubers of Paris polyphylla and Dioscorea opposita Thunb. "Jiao-ban Yam", respectively, differing by more than 9000-fold. These results suggest that the ELISA method can be used as a rapid, simple, sensitive, and accurate tool for quantitative analysis of samples containing diosgenin, and may provide an important criterion for quality evaluation and a valuable tool for quality control of diosgenin-containing medicinal plants.
关键词Diosgenin Elisa Hplc Medicinal Plants
DOI10.1055/s-0030-1250054
收录类别SCI
语种英语
WOS记录号WOS:000284144400026
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/23913
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Wuhan Univ, Coll Life Sci, Key Lab Minist Educ Plant Dev Biol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Peoples R China
推荐引用方式
GB/T 7714
Li, Jiaru,Yang, Dingyu,Yu, Kun,et al. Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay[J]. PLANTA MEDICA,2010,76(16):1915-1920.
APA Li, Jiaru,Yang, Dingyu,Yu, Kun,He, Ji,&Zhang, Yingjun.(2010).Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay.PLANTA MEDICA,76(16),1915-1920.
MLA Li, Jiaru,et al."Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay".PLANTA MEDICA 76.16(2010):1915-1920.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
s-0030-1250054.pdf(120KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Jiaru]的文章
[Yang, Dingyu]的文章
[Yu, Kun]的文章
百度学术
百度学术中相似的文章
[Li, Jiaru]的文章
[Yang, Dingyu]的文章
[Yu, Kun]的文章
必应学术
必应学术中相似的文章
[Li, Jiaru]的文章
[Yang, Dingyu]的文章
[Yu, Kun]的文章
相关权益政策
暂无数据
收藏/分享
文件名: s-0030-1250054.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。