| Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay | |
Li, Jiaru1; Yang, Dingyu1; Yu, Kun1; He, Ji1; Zhang, Yingjun2
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| 2010-11-01 | |
| 发表期刊 | PLANTA MEDICA
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| 卷号 | 76期号:16页码:1915-1920 |
| 摘要 | Many medicinal plants contain diosgenin, which has a significant medicinal value. However, there is currently no effective and rapid analytical method to determine the diosgenin content of plants or products. In the present work we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of diosgenin in herbal medicines. Diosgenin was conjugated with bovine serum albumin (BSA) for immunization. A polyclonal antibody developed in rabbits against a diosgenin-BSA conjugate was shown to be specific for diosgenin. The developed ELISA assay was highly sensitive, specific, and easy to perform. In addition, it gave more precise results with less variation than other methods that have been used in the past, including gravimetric and spectrophotometric assays, and correlated well with high-performance liquid chromatography. The diosgenin content determined by ELISA varied widely, with the highest and lowest values in rhizomes or tubers of Paris polyphylla and Dioscorea opposita Thunb. "Jiao-ban Yam", respectively, differing by more than 9000-fold. These results suggest that the ELISA method can be used as a rapid, simple, sensitive, and accurate tool for quantitative analysis of samples containing diosgenin, and may provide an important criterion for quality evaluation and a valuable tool for quality control of diosgenin-containing medicinal plants. |
| 关键词 | Diosgenin Elisa Hplc Medicinal Plants |
| DOI | 10.1055/s-0030-1250054 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284144400026 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/23913 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Wuhan Univ, Coll Life Sci, Key Lab Minist Educ Plant Dev Biol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jiaru,Yang, Dingyu,Yu, Kun,et al. Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay[J]. PLANTA MEDICA,2010,76(16):1915-1920. |
| APA | Li, Jiaru,Yang, Dingyu,Yu, Kun,He, Ji,&Zhang, Yingjun.(2010).Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay.PLANTA MEDICA,76(16),1915-1920. |
| MLA | Li, Jiaru,et al."Determination of Diosgenin Content in Medicinal Plants with Enzyme-Linked Immunosorbent Assay".PLANTA MEDICA 76.16(2010):1915-1920. |
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