Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design

doi:10.1021/jf073020u

	Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design
	Li, Peng ; Xu, Gang; Li, Shao-Ping ; Wang, Yi-Tao ; Fan, Tai-Ping ; Zhao, Qin-Shi; Zhang, Qing-Wen
	2008-02-27
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号	56 期号:4 页码:1164-1171
摘要	A rapid, sensitive, reproducible, and accurate ultraperformance liquid chromatographic (UPLC) method was developed for the simultaneous determination of 10 diterpenoid compounds (tanshinone 1, tanshinone IIA, cryptotanshinone, dihydrotanshinone I, 1,2-dihydrotanshinquinone, methylenetanshinquinone, miltirone, 5,6-dehydrosugiol, sugiol, and przewalskin) in Salvia miltiorrhiza for the first time. Central composite design was applied as a powerful tool to optimize the most dominant parameters that influence the resolution of UPLC, that is, gradient, flow rate, and column temperature. Under optimum conditions, all peaks except 1,2-dihydrotanshinquinone and methylenetanshinquinone could be baseline separated within 8 min. Furthermore, the contents of these compounds in S. miltiorrhiza samples collected from different provinces of China have also been compared. The results showed that UPLC is one of the most efficient methods for the analysis of diterpenoid compounds in S. miltiorrhiza and that it is a potential method for quality control of this valuable traditional Chinese medicine.
关键词	Ultraperformance Liquid Chromatography (Uplc) Diterpenoid Salvia Miltiorrhiza Central Composite Design
DOI	10.1021/jf073020u
收录类别	SCI
语种	英语
WOS记录号	WOS:000253321200002
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/23863
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Univ Macau, Inst Chinese Med Sci, Macao, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China 3.Univ Cambridge, Dept Pharmacol, Angiogenesis & Tradit Chinese Med Lab, Cambridge CB2 1PD, England
推荐引用方式 GB/T 7714	Li, Peng,Xu, Gang,Li, Shao-Ping,et al. Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2008,56(4):1164-1171.
APA	Li, Peng.,Xu, Gang.,Li, Shao-Ping.,Wang, Yi-Tao.,Fan, Tai-Ping.,...&Zhang, Qing-Wen.(2008).Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,56(4),1164-1171.
MLA	Li, Peng,et al."Optimizing ultra performance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 56.4(2008):1164-1171.

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