Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China
Zhao, Xing-Feng1; Sun, Wei-Bang1; Yang, Jun-Bo2; Meng, Jing2,3
2009-10-01
发表期刊CONSERVATION GENETICS
ISSN1566-0621
卷号10期号:5页码:1583-1585
摘要By using a modified biotin-streptavidin capture method, a total of 12 microsatellite loci were developed and characterized for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China. The number of alleles (A) ranged from two to six in 30 samples of this species. The ranges of observed (H(O)) and expected (H(E)) heterozygosities were 0.033-0.8000 and 0.033-0.7910, respectively. Cross-species amplification in M. yunnanensis showed that a subset of these markers holds promise for congeneric species. These polymorphic SSR markers would be useful tools for population genetics studies on M. coriacea and other congeneric species.
关键词Genetic Diversity Michelia Coriacea Population Structure Ssr Markers
资助信息This work was supported by a special fund of China's Yunnan Government for qualified scientists and technicians (grant number 2006PY-48 to W. Sun) and generous support of Botanical Gardens Conservation International (BGCI) (grant number 6206/R4331 to W. Sun). This study was conducted on the Key Laboratory of Plant Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences. The authors particularly thank Professor Eliane Norman of Stetson University in USA for her great help in editing the text.
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2349
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog, Kunming 650204, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Zhao, Xing-Feng,Sun, Wei-Bang,Yang, Jun-Bo,et al. Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China[J]. CONSERVATION GENETICS,2009,10(5):1583-1585.
APA Zhao, Xing-Feng,Sun, Wei-Bang,Yang, Jun-Bo,&Meng, Jing.(2009).Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China.CONSERVATION GENETICS,10(5),1583-1585.
MLA Zhao, Xing-Feng,et al."Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China".CONSERVATION GENETICS 10.5(2009):1583-1585.
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