Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)
Liao, Pei-Chun4; Gong, Xun3; Shih, Huei-Chuan2; Chiang, Yu-Chung1
2009-12-01
发表期刊CONSERVATION GENETICS
ISSN1566-0621
卷号10期号:6页码:1911-1914
摘要Piper polysyphonum is an endemic species in southeast Asia, in the narrow habitat located in the Chinese provinces of Guizhou and Yunnan, and the country of Laos. Recently, loss of forests due to agricultural activity has dramatically reduced the habitat and population size of P. polysyphonum. In this study, eleven primer sets of polymorphic microsatellite DNA loci were developed for P. polysyphonum. Allele numbers ranged from two to ten, with observed heterozygosities ranging from 0.222 to 0.889. Four loci exhibited a departure from Hardy-Weinberg equilibrium, possibly due to population admixture. No loci pairs revealed significant linkage disequilibrium. Among the eleven loci, two with extremely high numbers of TCG repeats were obtained. The polymorphic microsatellite DNA markers reported here should provide a helpful means to address questions concerning population structure and demographic history of P. polysyphonum for conservation efforts.
关键词Piper Polysyphonum Endemic Species Microsatellite Dna
资助信息This study was supported by grants from the National Science Council of the ROC on Taiwan awarded to Yu-Chung Chiang.
学科领域Biodiversity Conservation ; Genetics & Heredity
DOI10.1007/s10592-009-9852-x
收录类别SCI
语种英语
WOS研究方向Biodiversity & Conservation ; Genetics & Heredity
WOS类目Biodiversity Conservation ; Genetics & Heredity
WOS记录号WOS:000272374100045
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2345
专题资源植物与生物技术所级重点实验室
作者单位1.Natl Pingtung Univ Sci & Technol, Dept Life Sci, Neipu 912, Pingtung, Taiwan
2.Mei Ho Inst Technol, Sch Nursing, Pingtung 912, Taiwan
3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
4.Natl Taiwan Normal Univ, Dept Life Sci, Taipei 116, Taiwan
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Liao, Pei-Chun,Gong, Xun,Shih, Huei-Chuan,et al. Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)[J]. CONSERVATION GENETICS,2009,10(6):1911-1914.
APA Liao, Pei-Chun,Gong, Xun,Shih, Huei-Chuan,&Chiang, Yu-Chung.(2009).Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae).CONSERVATION GENETICS,10(6),1911-1914.
MLA Liao, Pei-Chun,et al."Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)".CONSERVATION GENETICS 10.6(2009):1911-1914.
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