Piper polysyphonum is an endemic species in southeast Asia, in the narrow habitat located in the Chinese provinces of Guizhou and Yunnan, and the country of Laos. Recently, loss of forests due to agricultural activity has dramatically reduced the habitat and population size of P. polysyphonum. In this study, eleven primer sets of polymorphic microsatellite DNA loci were developed for P. polysyphonum. Allele numbers ranged from two to ten, with observed heterozygosities ranging from 0.222 to 0.889. Four loci exhibited a departure from Hardy-Weinberg equilibrium, possibly due to population admixture. No loci pairs revealed significant linkage disequilibrium. Among the eleven loci, two with extremely high numbers of TCG repeats were obtained. The polymorphic microsatellite DNA markers reported here should provide a helpful means to address questions concerning population structure and demographic history of P. polysyphonum for conservation efforts.
1.Natl Pingtung Univ Sci & Technol, Dept Life Sci, Neipu 912, Pingtung, Taiwan 2.Mei Ho Inst Technol, Sch Nursing, Pingtung 912, Taiwan 3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 4.Natl Taiwan Normal Univ, Dept Life Sci, Taipei 116, Taiwan
Recommended Citation:
Liao, PC; Gong, X; Shih, HC; Chiang, YC.Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae),CONSERVATION GENETICS,2009,10(6):1911-1914
