Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)

doi:10.1007/s10592-009-9852-x

	Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)
	Liao, Pei-Chun 4; Gong, Xun3 ; Shih, Huei-Chuan 2; Chiang, Yu-Chung 1
	2009-12-01
发表期刊	CONSERVATION GENETICS
ISSN	1566-0621
卷号	10 期号:6 页码:1911-1914
摘要	Piper polysyphonum is an endemic species in southeast Asia, in the narrow habitat located in the Chinese provinces of Guizhou and Yunnan, and the country of Laos. Recently, loss of forests due to agricultural activity has dramatically reduced the habitat and population size of P. polysyphonum. In this study, eleven primer sets of polymorphic microsatellite DNA loci were developed for P. polysyphonum. Allele numbers ranged from two to ten, with observed heterozygosities ranging from 0.222 to 0.889. Four loci exhibited a departure from Hardy-Weinberg equilibrium, possibly due to population admixture. No loci pairs revealed significant linkage disequilibrium. Among the eleven loci, two with extremely high numbers of TCG repeats were obtained. The polymorphic microsatellite DNA markers reported here should provide a helpful means to address questions concerning population structure and demographic history of P. polysyphonum for conservation efforts.
关键词	Piper Polysyphonum Endemic Species Microsatellite Dna
资助信息	This study was supported by grants from the National Science Council of the ROC on Taiwan awarded to Yu-Chung Chiang.
收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2345
专题	资源植物与生物技术所级重点实验室
作者单位	1.Natl Pingtung Univ Sci & Technol, Dept Life Sci, Neipu 912, Pingtung, Taiwan 2.Mei Ho Inst Technol, Sch Nursing, Pingtung 912, Taiwan 3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 4.Natl Taiwan Normal Univ, Dept Life Sci, Taipei 116, Taiwan
推荐引用方式 GB/T 7714	Liao, Pei-Chun,Gong, Xun,Shih, Huei-Chuan,et al. Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)[J]. CONSERVATION GENETICS,2009,10(6):1911-1914.
APA	Liao, Pei-Chun,Gong, Xun,Shih, Huei-Chuan,&Chiang, Yu-Chung.(2009).Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae).CONSERVATION GENETICS,10(6),1911-1914.
MLA	Liao, Pei-Chun,et al."Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae)".CONSERVATION GENETICS 10.6(2009):1911-1914.

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