Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative
Xu,Xiongbo; Gu,Zixin; Liu,Shao; Gao,Na; He,Xiaozhen; Xin,Xiu; Liu,S (reprint author),Xiangya Hosp,Dept Pharm,Changsha 410008,Hunan,Peoples R China.; liushao999@hotmail.com
2015-09-05
Source PublicationCARBOHYDRATE POLYMERS
Volume128Pages:138-146
AbstractA water-soluble glucan, BCG-PASW, with a molecular weight of 2.10 x 10(4) Da, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an alpha-D-glucan composed of alpha-D-(1 -> 4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal alpha-D-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordBcg-psn Purification Characterization Sulfated Derivative Antitumor
Subject AreaChemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI10.1016/j.carbpol.2015.04.025
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000356209900016
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/21358
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorLiu,S (reprint author),Xiangya Hosp,Dept Pharm,Changsha 410008,Hunan,Peoples R China.; liushao999@hotmail.com
Recommended Citation
GB/T 7714
Xu,Xiongbo,Gu,Zixin,Liu,Shao,et al. Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative[J]. CARBOHYDRATE POLYMERS,2015,128:138-146.
APA Xu,Xiongbo.,Gu,Zixin.,Liu,Shao.,Gao,Na.,He,Xiaozhen.,...&liushao999@hotmail.com.(2015).Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative.CARBOHYDRATE POLYMERS,128,138-146.
MLA Xu,Xiongbo,et al."Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative".CARBOHYDRATE POLYMERS 128(2015):138-146.
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