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题名: Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative
作者: Xu,Xiongbo; Gu,Zixin; Liu,Shao; Gao,Na; He,Xiaozhen; Xin,Xiu
刊名: CARBOHYDRATE POLYMERS
出版日期: 2015-09-05
卷号: 128, 页码:138-146
关键词: BCG-PSN ; Purification ; Characterization ; Sulfated derivative ; Antitumor
学科分类: Chemistry, Applied; Chemistry, Organic; Polymer Science
DOI: 10.1016/j.carbpol.2015.04.025
通讯作者: Liu,S (reprint author),Xiangya Hosp,Dept Pharm,Changsha 410008,Hunan,Peoples R China. ; liushao999@hotmail.com
文章类型: Article
英文摘要: A water-soluble glucan, BCG-PASW, with a molecular weight of 2.10 x 10(4) Da, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an alpha-D-glucan composed of alpha-D-(1 -> 4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal alpha-D-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides. (C) 2015 Elsevier Ltd. All rights reserved.
类目[WOS]: Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]: Chemistry ; Polymer Science
关键词[WOS]: MAGNETIC-RESONANCE SPECTROSCOPY ; ANTIOXIDANT ACTIVITY ; IMMUNOSTIMULATORY ACTIVITY ; STRUCTURAL FEATURES ; RADIX-HEDYSARI ; POLYSACCHARIDE ; ANTIGLYCATION ; AMYGDALIN ; SINENSIS ; ROOTS
收录类别: SCI
项目资助者: Kunming Institute of Botany, Chinese Academy of Sciences, China ; Cancer Research Institute of Central South University, China
语种: 英语
WOS记录号: WOS:000356209900016
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/21358
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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