beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments
Gao, Na1,2; Lu, Feng3; Xiao, Chuang1; Yang, Lian1; Chen, Jun1; Zhou, Kai1; Wen, Dandan4; Li, Zi1; Wu, Mingyi1; Jiang, Jianmin4; Liu, Guangming1,3; Zhao, Jinhua1; Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China.; lgm13330555378@126.com; zhao.jinhua@yahoo.com
2015-08-20
Source PublicationCARBOHYDRATE POLYMERS
Volume127Pages:427-437
AbstractFucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for beta-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GaINAc-beta 1,4-GlcA were selectively cleaved and distinctive Delta(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with M-w 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordFucosylated Chondroitin Sulfate Benzyl Esterification Beta-elimination Depolymerization Anticoagulant
Subject AreaChemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI10.1016/j.carbpol.2015.04.002
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000355707500053
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/21341
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorLiu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China.; lgm13330555378@126.com; zhao.jinhua@yahoo.com
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dali Univ, Coll Pharm & Chem, Dali, Peoples R China
4.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510275, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Gao, Na,Lu, Feng,Xiao, Chuang,et al. beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments[J]. CARBOHYDRATE POLYMERS,2015,127:427-437.
APA Gao, Na.,Lu, Feng.,Xiao, Chuang.,Yang, Lian.,Chen, Jun.,...&zhao.jinhua@yahoo.com.(2015).beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments.CARBOHYDRATE POLYMERS,127,427-437.
MLA Gao, Na,et al."beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments".CARBOHYDRATE POLYMERS 127(2015):427-437.
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