beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments

doi:10.1016/j.carbpol.2015.04.002

	beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments
	Gao, Na 1,2; Lu, Feng 3; Xiao, Chuang1 ; Yang, Lian 1; Chen, Jun 1; Zhou, Kai 1; Wen, Dandan 4; Li, Zi 1; Wu, Mingyi 1; Jiang, Jianmin 4; Liu, Guangming 1,3; Zhao, Jinhua1 ; Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China.; lgm13330555378@126.com ; zhao.jinhua@yahoo.com
	2015-08-20
发表期刊	CARBOHYDRATE POLYMERS
卷号	127 页码:427-437
摘要	Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for beta-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GaINAc-beta 1,4-GlcA were selectively cleaved and distinctive Delta(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with M-w 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Fucosylated Chondroitin Sulfate Benzyl Esterification Beta-elimination Depolymerization Anticoagulant
学科领域	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI	10.1016/j.carbpol.2015.04.002
收录类别	SCI
语种	英语
WOS记录号	WOS:000355707500053
引用统计	被引频次：57[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/21341
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China.; lgm13330555378@126.com; zhao.jinhua@yahoo.com
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Dali Univ, Coll Pharm & Chem, Dali, Peoples R China 4.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Gao, Na,Lu, Feng,Xiao, Chuang,et al. beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments[J]. CARBOHYDRATE POLYMERS,2015,127:427-437.
APA	Gao, Na.,Lu, Feng.,Xiao, Chuang.,Yang, Lian.,Chen, Jun.,...&zhao.jinhua@yahoo.com.(2015).beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments.CARBOHYDRATE POLYMERS,127,427-437.
MLA	Gao, Na,et al."beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments".CARBOHYDRATE POLYMERS 127(2015):427-437.

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