中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 植物化学与西部植物资源持续利用国家重点实验室  > 期刊论文
题名: beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments
作者: Gao, Na1, 2; Lu, Feng3; Xiao, Chuang1; Yang, Lian1; Chen, Jun1; Zhou, Kai1; Wen, Dandan4; Li, Zi1; Wu, Mingyi1; Jiang, Jianmin4; Liu, Guangming1, 3; Zhao, Jinhua1
刊名: CARBOHYDRATE POLYMERS
出版日期: 2015-08-20
卷号: 127, 页码:427-437
关键词: Fucosylated chondroitin sulfate ; Benzyl esterification ; beta-Elimination ; Depolymerization ; Anticoagulant
学科分类: Chemistry, Applied; Chemistry, Organic; Polymer Science
DOI: 10.1016/j.carbpol.2015.04.002
通讯作者: Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China. ; lgm13330555378@126.com ; zhao.jinhua@yahoo.com
文章类型: Article
英文摘要: Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for beta-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GaINAc-beta 1,4-GlcA were selectively cleaved and distinctive Delta(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with M-w 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. (C) 2015 Elsevier Ltd. All rights reserved.
类目[WOS]: Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]: Chemistry ; Polymer Science
关键词[WOS]: STICHOPUS-JAPONICUS SELENKA ; SEA-CUCUMBER ; BODY-WALL ; GLYCOSAMINOGLYCAN ; HEPARIN ; POLYSACCHARIDES ; BINDING
收录类别: SCI
项目资助者: Yunnan Provincial Science and Technology Department in China [2010CI116, 2012FB177, 2013FA046] ; National Natural Science Foundation of China [81102372, 81373292] ; Outstanding Technical Talent Foundation of the Chinese Academy of Sciences ; West Light Foundation of the Chinese Academy of Sciences
语种: 英语
WOS记录号: WOS:000355707500053
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/21341
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
gao2015.pdf(2985KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dali Univ, Coll Pharm & Chem, Dali, Peoples R China
4.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510275, Guangdong, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, Na]'s Articles
[Lu, Feng]'s Articles
[Xiao, Chuang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, Na]‘s Articles
[Lu, Feng]‘s Articles
[Xiao, Chuang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: gao2015.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace