beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments
Gao, Na1,2; Lu, Feng3; Xiao, Chuang1; Yang, Lian1; Chen, Jun1; Zhou, Kai1; Wen, Dandan4; Li, Zi1; Wu, Mingyi1; Jiang, Jianmin4; Liu, Guangming1,3; Zhao, Jinhua1; Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China.; lgm13330555378@126.com; zhao.jinhua@yahoo.com
2015-08-20
发表期刊CARBOHYDRATE POLYMERS
卷号127页码:427-437
摘要Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for beta-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GaINAc-beta 1,4-GlcA were selectively cleaved and distinctive Delta(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with M-w 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. (C) 2015 Elsevier Ltd. All rights reserved.
关键词Fucosylated Chondroitin Sulfate Benzyl Esterification Beta-elimination Depolymerization Anticoagulant
资助信息Yunnan Provincial Science and Technology Department in China [2010CI116, 2012FB177, 2013FA046]; National Natural Science Foundation of China [81102372, 81373292]; Outstanding Technical Talent Foundation of the Chinese Academy of Sciences; West Light Foundation of the Chinese Academy of Sciences
学科领域Chemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI10.1016/j.carbpol.2015.04.002
收录类别SCI
语种英语
WOS研究方向Chemistry ; Polymer Science
WOS类目Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号WOS:000355707500053
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/21341
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China.; lgm13330555378@126.com; zhao.jinhua@yahoo.com
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dali Univ, Coll Pharm & Chem, Dali, Peoples R China
4.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510275, Guangdong, Peoples R China
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GB/T 7714
Gao, Na,Lu, Feng,Xiao, Chuang,et al. beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments[J]. CARBOHYDRATE POLYMERS,2015,127:427-437.
APA Gao, Na.,Lu, Feng.,Xiao, Chuang.,Yang, Lian.,Chen, Jun.,...&zhao.jinhua@yahoo.com.(2015).beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments.CARBOHYDRATE POLYMERS,127,427-437.
MLA Gao, Na,et al."beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments".CARBOHYDRATE POLYMERS 127(2015):427-437.
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