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题名: An endophytic Coniochaeta velutina producing broad spectrum antimycotics
作者: Xie, Jie1; Strobel, Gary A.2; Feng, Tao3; Ren, Huishuang1; Mends, Morgan T.2; Zhou, Zeyang1; Geary, Brad4
刊名: JOURNAL OF MICROBIOLOGY
出版日期: 2015-06-01
卷号: 53, 期号:6, 页码:390-397
关键词: antimycotics ; endophyte ; Coniochaeta velutina ; bioassays ; SEM
学科分类: Microbiology
DOI: 10.1007/s12275-015-5105-5
通讯作者: Strobel,GA (reprint author),Montana State Univ,Dept Plant Sci,Bozeman,MT 59717 USA. ; uplgs@montana.edu
文章类型: Article
英文摘要: An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 mu g/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non-biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol - anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: MUSCODOR-ALBUS ; FUNGUS ; METABOLITES ; GLYCOSIDES ; GEN. ; NMR ; NOV
收录类别: SCI
项目资助者: Open Foundation of State Key Laboratory of Silkworm Genome Biology, China [SKLSGB2013025] ; Special Research Foundation of Southwest University in China [XDJK2014C155]
语种: 英语
WOS记录号: WOS:000355344600005
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/21334
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Southwest Univ, Coll Biotechnol, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China
2.Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
4.Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA
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