An endophytic Coniochaeta velutina producing broad spectrum antimycotics
Xie, Jie1; Strobel, Gary A.2; Feng, Tao3; Ren, Huishuang1; Mends, Morgan T.2; Zhou, Zeyang1; Geary, Brad4; Strobel,GA (reprint author),Montana State Univ,Dept Plant Sci,Bozeman,MT 59717 USA.; uplgs@montana.edu
2015-06-01
发表期刊JOURNAL OF MICROBIOLOGY
卷号53期号:6页码:390-397
摘要An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 mu g/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non-biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol - anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.
关键词Antimycotics Endophyte Coniochaeta Velutina Bioassays Sem
资助信息Open Foundation of State Key Laboratory of Silkworm Genome Biology, China [SKLSGB2013025]; Special Research Foundation of Southwest University in China [XDJK2014C155]
学科领域Microbiology
DOI10.1007/s12275-015-5105-5
收录类别SCI
语种英语
WOS研究方向Microbiology
WOS类目Microbiology
WOS记录号WOS:000355344600005
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/21334
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Strobel,GA (reprint author),Montana State Univ,Dept Plant Sci,Bozeman,MT 59717 USA.; uplgs@montana.edu
作者单位1.Southwest Univ, Coll Biotechnol, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China
2.Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
4.Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA
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Xie, Jie,Strobel, Gary A.,Feng, Tao,et al. An endophytic Coniochaeta velutina producing broad spectrum antimycotics[J]. JOURNAL OF MICROBIOLOGY,2015,53(6):390-397.
APA Xie, Jie.,Strobel, Gary A..,Feng, Tao.,Ren, Huishuang.,Mends, Morgan T..,...&uplgs@montana.edu.(2015).An endophytic Coniochaeta velutina producing broad spectrum antimycotics.JOURNAL OF MICROBIOLOGY,53(6),390-397.
MLA Xie, Jie,et al."An endophytic Coniochaeta velutina producing broad spectrum antimycotics".JOURNAL OF MICROBIOLOGY 53.6(2015):390-397.
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