Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl

doi:10.1007/s00299-006-0226-2

	Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl
	Wang, Hao-Xin ; Yang, Tao ; Zeng, Ying; Hu, Zhong
	2007-03-01
发表期刊	PLANT CELL REPORTS
ISSN	0721-7714
卷号	26 期号:3 页码:253-259
摘要	Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiological functions in the plant are rarely understood. Aspects of expression of gastrodianins in growth and development of the plant will be helpful to dissect their functional roles. Two types of cDNA clones with complete cDNA sequences matching the known gastrodianins were obtained from G. elata Bl.f.glauca S.chow (Orchidaceae) and designated gastrodianin-4A (ga4A) and gastrodianin-4B (ga4B), respectively. But only one isoform was found to be expressed in all different parts of a single plant. Based on the RNA gel blot analysis, gastrodianins were much more abundantly expressed in the fully opened flowers than the underground corms where an enhanced expression was found in the out layers of secondary corms. By RNA in situ hybridization gastrodianin transcripts were distinctly detected in the cortical cells and vascular cells of corms. Strong transcript accumulations were observed in two to eight layers of cortical cells in secondary corms. From its peripheral tissue expression pattern and level in corms and flowers, the gastrodianin may account for a possible defense against phytopathogens or insects.
关键词	Armillaria Mellea Gastrodia Antifungal Protein Gastrodia Elata Gastrodianins Mannose-binding Protein Orchid
学科领域	Plant Sciences
DOI	10.1007/s00299-006-0226-2
收录类别	SCI
语种	英语
WOS记录号	WOS:000244200500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2111
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hao-Xin,Yang, Tao,Zeng, Ying,et al. Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl[J]. PLANT CELL REPORTS,2007,26(3):253-259.
APA	Wang, Hao-Xin,Yang, Tao,Zeng, Ying,&Hu, Zhong.(2007).Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl.PLANT CELL REPORTS,26(3),253-259.
MLA	Wang, Hao-Xin,et al."Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl".PLANT CELL REPORTS 26.3(2007):253-259.

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