Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl
Wang, Hao-Xin; Yang, Tao; Zeng, Ying; Hu, Zhong
2007-03-01
发表期刊PLANT CELL REPORTS
ISSN0721-7714
卷号26期号:3页码:253-259
摘要Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiological functions in the plant are rarely understood. Aspects of expression of gastrodianins in growth and development of the plant will be helpful to dissect their functional roles. Two types of cDNA clones with complete cDNA sequences matching the known gastrodianins were obtained from G. elata Bl.f.glauca S.chow (Orchidaceae) and designated gastrodianin-4A (ga4A) and gastrodianin-4B (ga4B), respectively. But only one isoform was found to be expressed in all different parts of a single plant. Based on the RNA gel blot analysis, gastrodianins were much more abundantly expressed in the fully opened flowers than the underground corms where an enhanced expression was found in the out layers of secondary corms. By RNA in situ hybridization gastrodianin transcripts were distinctly detected in the cortical cells and vascular cells of corms. Strong transcript accumulations were observed in two to eight layers of cortical cells in secondary corms. From its peripheral tissue expression pattern and level in corms and flowers, the gastrodianin may account for a possible defense against phytopathogens or insects.
关键词Armillaria Mellea Gastrodia Antifungal Protein Gastrodia Elata Gastrodianins Mannose-binding Protein Orchid
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2111
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Wang, Hao-Xin,Yang, Tao,Zeng, Ying,et al. Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl[J]. PLANT CELL REPORTS,2007,26(3):253-259.
APA Wang, Hao-Xin,Yang, Tao,Zeng, Ying,&Hu, Zhong.(2007).Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl.PLANT CELL REPORTS,26(3),253-259.
MLA Wang, Hao-Xin,et al."Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl".PLANT CELL REPORTS 26.3(2007):253-259.
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