Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes
Luo, Hong; Liu, Yajun; Fang, Lin; Li, Xuan; Tang, Ninghua; Zhang, Keqin
2007-06-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume73Issue:12Pages:3916-3923
AbstractWe reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of Coprinus comatus (O. F. Mull.:Fr.) Pers. Although some observations regarding the role of this structure were presented, its function remained largely unknown. In this study, we showed that purified (isolated and washed) spiny balls could immobilize and kill the free-living nematode Panagrellus redivivus Goodey highly efficiently. Scanning electron microscopy studies illustrated that the spiny structure damaged the nematode cuticle, suggesting the presence of a mechanical force during the process of nematode immobilization. Severe injuries on nematode cuticles caused the leakage of inner materials of the nematodes. When these structures were ground in liquid nitrogen, their killing efficacy against nematodes was lost, indicating that the shape and the complete structure of the spiny balls are indispensable for their function. However, extraction with organic solvents never lowered their activity against P. redivivus, and the extracts showed no obvious effect on the nematode. We also investigated whether C comatus was able to produce toxins which would aid in the immobilization of nematodes. In total, we identified seven toxins from C. comatus that showed activity to immobilize the nematodes P. redivivus and Meloidogyne incognita (Kofoid et White) Chitwood. The chemical structures of these toxins were identified with nuclear magnetic resonance, mass spectrometry, infrared, and LTV spectrum analysis. Two compounds were found to be novel. The toxins found in C. comatus are O-containing heterocyclic compounds.
KeywordBiological-activities Nematophagous Fungi Stephanocysts Nematoctonus Metabolites
Subject AreaBiotechnology & Applied Microbiology ; Microbiology
DOI10.1128/AEM.02770-06
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000247394200018
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/2071
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Yunnan Univ, Lab Conservat & Utilizat Bioresources, Yunnan 650091, Peoples R China
2.Yunnan Normal Univ, Middle Sch Attached, Yunnan 650106, Peoples R China
3.Kunming Univ Sci & Technol, Fac Environm Sci & Engn, Kunming 650093, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
Recommended Citation
GB/T 7714
Luo, Hong,Liu, Yajun,Fang, Lin,et al. Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2007,73(12):3916-3923.
APA Luo, Hong,Liu, Yajun,Fang, Lin,Li, Xuan,Tang, Ninghua,&Zhang, Keqin.(2007).Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,73(12),3916-3923.
MLA Luo, Hong,et al."Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73.12(2007):3916-3923.
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