| Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd | |
Zhang, Qian; Liu, Hongwei; Sun, Guiling ; Wilson, Iain W.; Wu, Jianqiang; Hoffman, Angela; Cheng, Junwen; Qiu, Deyou; Qiu,DY (reprint author),Chinese Acad Forestry,Res Inst Forestry,State Key Lab Tree Genet & Breeding,Beijing 100091,Peoples R China.; peterqdy@aliyun.com
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| 2015-03-30 | |
| Source Publication | PLOS ONE
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| Volume | 10Issue:3Pages:e0123026 |
| Abstract | Taxol (paclitaxel) a diterpenoid is one of the most effective anticancer drugs identified. Biosynthesis of taxol was considered restricted to the Taxus genera until Stierle et al. discovered that an endophytic fungus isolated from Taxus brevifolia could independently synthesize taxol. Little is known about the mechanism of taxol biosynthesis in microbes, but it has been speculated that its biosynthesis may differ from plants. The microbiome from the roots of Taxus chinensis have been extensively investigated with culture-dependent methods to identify taxol synthesizing microbes, but not using culture independent methods., Using bar-coded high-throughput sequencing in combination with a metagenomics approach, we surveyed the microbial diversity and gene composition of the root-associated microbiomefrom Taxus chinensis (Pilger) Rehd. High-throughput amplicon sequencing revealed 187 fungal OTUs which is higher than any previously reported fungal number identified with the culture-dependent method, suggesting that T. chinensis roots harbor novel and diverse fungi. Some operational taxonomic units (OTU) identified were identical to reported microbe strains possessing the ability to synthesis taxol and several genes previously associated with taxol biosynthesis were identified through metagenomics analysis. |
| Subject Area | Multidisciplinary Sciences |
| DOI | 10.1371/journal.pone.0123026 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000352134700212 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/20572 |
| Collection | 资源植物与生物技术所级重点实验室 |
| Corresponding Author | Qiu,DY (reprint author),Chinese Acad Forestry,Res Inst Forestry,State Key Lab Tree Genet & Breeding,Beijing 100091,Peoples R China.; peterqdy@aliyun.com |
| Recommended Citation GB/T 7714 | Zhang, Qian,Liu, Hongwei,Sun, Guiling,et al. Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd[J]. PLOS ONE,2015,10(3):e0123026. |
| APA | Zhang, Qian.,Liu, Hongwei.,Sun, Guiling.,Wilson, Iain W..,Wu, Jianqiang.,...&peterqdy@aliyun.com.(2015).Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd.PLOS ONE,10(3),e0123026. |
| MLA | Zhang, Qian,et al."Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd".PLOS ONE 10.3(2015):e0123026. |
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| asset1.pdf（1056KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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