Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd

doi:10.1371/journal.pone.0123026

	Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd
	Zhang, Qian; Liu, Hongwei; Sun, Guiling; Wilson, Iain W.; Wu, Jianqiang; Hoffman, Angela; Cheng, Junwen; Qiu, Deyou; Qiu,DY (reprint author),Chinese Acad Forestry,Res Inst Forestry,State Key Lab Tree Genet & Breeding,Beijing 100091,Peoples R China.; peterqdy@aliyun.com
	2015-03-30
发表期刊	PLOS ONE
卷号	10 期号:3 页码:e0123026
摘要	Taxol (paclitaxel) a diterpenoid is one of the most effective anticancer drugs identified. Biosynthesis of taxol was considered restricted to the Taxus genera until Stierle et al. discovered that an endophytic fungus isolated from Taxus brevifolia could independently synthesize taxol. Little is known about the mechanism of taxol biosynthesis in microbes, but it has been speculated that its biosynthesis may differ from plants. The microbiome from the roots of Taxus chinensis have been extensively investigated with culture-dependent methods to identify taxol synthesizing microbes, but not using culture independent methods., Using bar-coded high-throughput sequencing in combination with a metagenomics approach, we surveyed the microbial diversity and gene composition of the root-associated microbiomefrom Taxus chinensis (Pilger) Rehd. High-throughput amplicon sequencing revealed 187 fungal OTUs which is higher than any previously reported fungal number identified with the culture-dependent method, suggesting that T. chinensis roots harbor novel and diverse fungi. Some operational taxonomic units (OTU) identified were identical to reported microbe strains possessing the ability to synthesis taxol and several genes previously associated with taxol biosynthesis were identified through metagenomics analysis.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0123026
收录类别	SCI
语种	英语
WOS记录号	WOS:000352134700212
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/20572
专题	资源植物与生物技术所级重点实验室
通讯作者	Qiu,DY (reprint author),Chinese Acad Forestry,Res Inst Forestry,State Key Lab Tree Genet & Breeding,Beijing 100091,Peoples R China.; peterqdy@aliyun.com
推荐引用方式 GB/T 7714	Zhang, Qian,Liu, Hongwei,Sun, Guiling,et al. Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd[J]. PLOS ONE,2015,10(3):e0123026.
APA	Zhang, Qian.,Liu, Hongwei.,Sun, Guiling.,Wilson, Iain W..,Wu, Jianqiang.,...&peterqdy@aliyun.com.(2015).Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd.PLOS ONE,10(3),e0123026.
MLA	Zhang, Qian,et al."Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd".PLOS ONE 10.3(2015):e0123026.

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