中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 资源植物与生物技术所级重点实验室  > 期刊论文
题名: Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau
作者: Ma,Lan ; Sun,Xuolong ; Kong,Xiangxiang ; Valero Galvan,Jose ; Li X(李雄) ; Yang,Shihai ; Yang,Yunqiang ; Yang Yongping(杨永平) ; null(胡向阳)
刊名: JOURNAL OF PROTEOMICS
出版日期: 2015
卷号: 112, 页码:63-82
关键词: Comparative proteomics ; Potentilla saundersiana ; Adaptation
学科分类: Biochemical Research Methods
DOI: 10.1016/j.jprot.2014.08.009
通讯作者: Yang,YP (reprint author),Chinese Acad Sci,Plant Germplasm & Genom Ctr,Kunming Inst Bot,Germplasm Bank Wild Species,Kunming 650201,Yunnan,Peoples R China. ; malan@mail.kib.ac.cn ; sunxudong@mail.kib.ac.cn ; kongxiangxiang@mail.kib.ac.cn ; jose.valero@uacj.mx ; lixiong@mail.kib.ac.cn ; yangshihai@mail.kib.ac.cn ; yangyunqiang@mail.kib.ac.cn ; yangyp@mail.kib.ac.cn ; huxiangyang@mail.kib.ac.cn
文章类型: Article
英文摘要: This study presents an analysis of leave and rood morphology, biochemical and proteomics approach as adaptation strategies of the alpine plant Potentilla saundersiana in an altitude gradient. Several plant physiological parameter, including root and leaf architecture, leaf photosynthesis capacity, specific leaf area (SLA) and leaf nitrogen concentration, histology and microscopy, anthocyanin and praline contents, antioxidant enzyme activity assay, in-gel enzyme activity staining, H2O2 and O-2(-) content, immunoblotting, auxin and strigolactone content and proteomics analysis were evaluated at five different altitudes. P. saundersiana modulated the root architecture and leaf phenotype to enhance adaptation to alpine environmental stress through mechanisms that involved hormone synthesis and signal transduction, particularly the cross-talk between auxin and strigolactone. Furthermore, an increase of antioxidant proteins and primary metabolites as a response to the alpine environment in P. saundersiana was observed. Proteins associated with the epigenetic regulation of DNA stability and post-translational protein degradation was also involved in this process. Based on these findings, P. saundersiana uses multiple strategies to adapt to the high-altitude environment of the Alpine region. Biological significance The alpine environment, which is characterized by sharp temperature shifts, high levels of ultraviolet radiation exposure, and low oxygen content, limits plant growth and distribution. Alpine plants have evolved strategies to survive the extremely harsh conditions prevailing at high altitudes; however, the underlying mechanisms remain poorly understood. The alpine plant Potentilla saundersiana is widespread in the Northwestern Tibetan Plateau. Here we adopted a comparative proteomics approach to investigate the mechanisms by which P. saundersiana withstands the alpine environment by examining plants located at five different altitudes. We detected and functionally characterized 118 proteins spots with variable abundance. Proteins involved in antioxidant activity, primary metabolites, epigenetic regulation, and protein post-translational modification play important roles in conferring tolerance to alpine environments. Furthermore, our results indicate that P. saundersiana modulates the root architecture and leaf phenotype to enhance adaptation to alpine environmental stress. These results provide novel insight into the multiple strategies underlying P. saundersiana adaptation to the high-altitude environment of the Northwestern Tibetan Plateau. (C) 2014 Elsevier B.V. All rights reserved.
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: ABIOTIC STRESS ; CROSS-TALK ; LEAF-AREA ; TRANSCRIPTOME ; BIOSYNTHESIS ; ARABIDOPSIS ; TOLERANCE ; RESPONSES ; STRIGOLACTONES ; ACCUMULATION
收录类别: SCI
项目资助者: Young Academic and Technical Leader Raising Foundation of Yunnan Province [2012HB041] ; National Natural Sciences Foundation of China [31170256] ; Major State Basic Research Development Program [2010CB951700]
语种: 英语
WOS记录号: WOS:000347021100006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/20564
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau.pdf(3075KB)----开放获取View Download
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma,Lan]'s Articles
[Sun,Xuolong]'s Articles
[Kong,Xiangxiang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma,Lan]‘s Articles
[Sun,Xuolong]‘s Articles
[Kong,Xiangxiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace