Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau
Ma,Lan; Sun,Xuolong; Kong,Xiangxiang; Valero Galvan,Jose; Li,Xiong; Yang,Shihai; Yang,Yunqiang; Yang,Yongping; Hu,Xiangyang; Yang,YP (reprint author),Chinese Acad Sci,Plant Germplasm & Genom Ctr,Kunming Inst Bot,Germplasm Bank Wild Species,Kunming 650201,Yunnan,Peoples R China.; malan@mail.kib.ac.cn; sunxudong@mail.kib.ac.cn; kongxiangxiang@mail.kib.ac.cn; jose.valero@uacj.mx; lixiong@mail.kib.ac.cn; yangshihai@mail.kib.ac.cn; yangyunqiang@mail.kib.ac.cn; yangyp@mail.kib.ac.cn; huxiangyang@mail.kib.ac.cn
2015
发表期刊JOURNAL OF PROTEOMICS
卷号112页码:63-82
摘要This study presents an analysis of leave and rood morphology, biochemical and proteomics approach as adaptation strategies of the alpine plant Potentilla saundersiana in an altitude gradient. Several plant physiological parameter, including root and leaf architecture, leaf photosynthesis capacity, specific leaf area (SLA) and leaf nitrogen concentration, histology and microscopy, anthocyanin and praline contents, antioxidant enzyme activity assay, in-gel enzyme activity staining, H2O2 and O-2(-) content, immunoblotting, auxin and strigolactone content and proteomics analysis were evaluated at five different altitudes. P. saundersiana modulated the root architecture and leaf phenotype to enhance adaptation to alpine environmental stress through mechanisms that involved hormone synthesis and signal transduction, particularly the cross-talk between auxin and strigolactone. Furthermore, an increase of antioxidant proteins and primary metabolites as a response to the alpine environment in P. saundersiana was observed. Proteins associated with the epigenetic regulation of DNA stability and post-translational protein degradation was also involved in this process. Based on these findings, P. saundersiana uses multiple strategies to adapt to the high-altitude environment of the Alpine region. Biological significance The alpine environment, which is characterized by sharp temperature shifts, high levels of ultraviolet radiation exposure, and low oxygen content, limits plant growth and distribution. Alpine plants have evolved strategies to survive the extremely harsh conditions prevailing at high altitudes; however, the underlying mechanisms remain poorly understood. The alpine plant Potentilla saundersiana is widespread in the Northwestern Tibetan Plateau. Here we adopted a comparative proteomics approach to investigate the mechanisms by which P. saundersiana withstands the alpine environment by examining plants located at five different altitudes. We detected and functionally characterized 118 proteins spots with variable abundance. Proteins involved in antioxidant activity, primary metabolites, epigenetic regulation, and protein post-translational modification play important roles in conferring tolerance to alpine environments. Furthermore, our results indicate that P. saundersiana modulates the root architecture and leaf phenotype to enhance adaptation to alpine environmental stress. These results provide novel insight into the multiple strategies underlying P. saundersiana adaptation to the high-altitude environment of the Northwestern Tibetan Plateau. (C) 2014 Elsevier B.V. All rights reserved.
关键词Comparative Proteomics Potentilla Saundersiana Adaptation
资助信息Young Academic and Technical Leader Raising Foundation of Yunnan Province [2012HB041]; National Natural Sciences Foundation of China [31170256]; Major State Basic Research Development Program [2010CB951700]
学科领域Biochemical Research Methods
DOI10.1016/j.jprot.2014.08.009
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology
WOS记录号WOS:000347021100006
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/20564
专题资源植物与生物技术所级重点实验室
通讯作者Yang,YP (reprint author),Chinese Acad Sci,Plant Germplasm & Genom Ctr,Kunming Inst Bot,Germplasm Bank Wild Species,Kunming 650201,Yunnan,Peoples R China.; malan@mail.kib.ac.cn; sunxudong@mail.kib.ac.cn; kongxiangxiang@mail.kib.ac.cn; jose.valero@uacj.mx; lixiong@mail.kib.ac.cn; yangshihai@mail.kib.ac.cn; yangyunqiang@mail.kib.ac.cn; yangyp@mail.kib.ac.cn; huxiangyang@mail.kib.ac.cn
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Ma,Lan,Sun,Xuolong,Kong,Xiangxiang,et al. Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau[J]. JOURNAL OF PROTEOMICS,2015,112:63-82.
APA Ma,Lan.,Sun,Xuolong.,Kong,Xiangxiang.,Valero Galvan,Jose.,Li,Xiong.,...&huxiangyang@mail.kib.ac.cn.(2015).Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau.JOURNAL OF PROTEOMICS,112,63-82.
MLA Ma,Lan,et al."Physiological, biochemical and proteomics analysis reveals the adaptation strategies of the alpine plant Potentilla saundersiana at altitude gradient of the Northwestern Tibetan Plateau".JOURNAL OF PROTEOMICS 112(2015):63-82.
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