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DNA barcoding of Nyssaceae (Cornales) and taxonomic issues
Wang, Nian1,2; Jacques, Frederic M. B.3; Milne, Richard I.4; Zhang, Chang-Qin1,2; Yang, Jun-Bo5
2012-04-01
Source PublicationBOTANICAL STUDIES
Volume53Issue:2Pages:265-274
AbstractDNA barcoding, as a tool for species discrimination, has been efficiently used in animals. However, there are still debates on which DNA region (s) can be adopted as the standard barcode (s) for land plants. In the present study, we evaluated the four proposed barcoding loci (matK, rbcL, trnH-psbA and ITS) on nine species of Nyssaceae. The results showed that ITS was the best performing single locus, although matK + rbcL might be used as the core barcodes for land plants. The chloroplast regions have low resolution compared with ITS. The low efficiency of these candidate barcodes in Nyssaceae might be caused by a poor taxonomy, especially within the genus Nyssa. The results also indicated that species status of N. shangszeensis, N. sinensis, N. shweliensis and N. wenshanensis requires to be reevaluated based on more morphological characters combined with rapidly evolving loci.
KeywordDna Barcoding Its Matk Nyssaceae Rbcl Trnh-psba
Indexed BySCI
Language英语
WOS IDWOS:000304577400011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/20457
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming Bot Garden, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Resource Plants & Biotechnol, Kunming 650204, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla 666303, Peoples R China
4.Univ Edinburgh, Inst Mol Plant Sci, Edinburgh EH9 3JH, Midlothian, Scotland
5.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Wang, Nian,Jacques, Frederic M. B.,Milne, Richard I.,et al. DNA barcoding of Nyssaceae (Cornales) and taxonomic issues[J]. BOTANICAL STUDIES,2012,53(2):265-274.
APA Wang, Nian,Jacques, Frederic M. B.,Milne, Richard I.,Zhang, Chang-Qin,&Yang, Jun-Bo.(2012).DNA barcoding of Nyssaceae (Cornales) and taxonomic issues.BOTANICAL STUDIES,53(2),265-274.
MLA Wang, Nian,et al."DNA barcoding of Nyssaceae (Cornales) and taxonomic issues".BOTANICAL STUDIES 53.2(2012):265-274.
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