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题名: Natural Diterpenoid Compound Elevates Expression of Bim Protein, Which Interacts with Antiapoptotic Protein Bcl-2, Converting It to Proapoptotic Bax-like Molecule
作者: Zhao, Lixia2, 3; He, Feng4; Liu, Haiyang1; Zhu, Yushan2, 3; Tian, Weili2, 3; Gao, Ping2, 3; He, Hongping1; Yue, Wen2, 3; Lei, Xiaobo2, 3; Ni, Biyun2, 3; Wang, Xiaohui2, 3; Jin, Haijing2, 3; Hao, Xiaojiang1; Lin, Jialing4; Chen, Quan2, 3
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期: 2012-01-06
卷号: 287, 期号:2, 页码:1054-1065
DOI: 10.1074/jbc.M111.264481
文章类型: Article
英文摘要: Overwhelming evidence indicates that Bax and Bak are indispensable for mediating cytochrome c release from mitochondria during apoptosis. Here we report a Bax/Bak-independent mechanism of cytochrome c release and apoptosis. We identified a natural diterpenoid compound that induced apoptosis in bax/bak double knock-out murine embryonic fibroblasts and substantially reduced the tumor growth from these cells implanted in mice. Treatment with the compound significantly increased expression of Bim, which migrated to mitochondria, altering the conformation of and forming oligomers with resident Bcl-2 to induce cytochrome c release and caspase activation. Importantly, purified Bim and Bcl-2 proteins cooperated to permeabilize a model mitochondrial outer membrane; this was accompanied by oligomerization of these proteins and deep embedding of Bcl-2 in the membrane. Therefore, the diterpenoid compound induces a structural and functional conversion of Bcl-2 through Bim to permeabilize the mitochondrial outer membrane, thereby inducing apoptosis independently of Bax and Bak. Because Bcl-2 family proteins play important roles in cancer development and relapse, this novel cell death mechanism can be explored for developing more effective anticancer therapeutics.
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: CELL-DEATH ; MEMBRANE PERMEABILIZATION ; MITOCHONDRIAL DYSFUNCTION ; TRIGGER APOPTOSIS ; BH3-ONLY PROTEINS ; BH3 DOMAIN ; FAMILY ; ACTIVATION ; BINDING ; CANCER
收录类别: SCI
语种: 英语
WOS记录号: WOS:000299170300022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/20452
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
2.Chinese Acad Sci, State Key Lab Biomembrane & Membrane Biotechnol, Inst Zool, Joint Lab Apoptosis & Mitochondrial Biol, Beijing 100101, Peoples R China
3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
4.Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73126 USA
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