Four Ligularia individuals possessing morphological characters of both L. nelumbifolia and L. subspicata were discovered in Shangrila County, Yunnan Province, China. DNA sequencing showed that the specimens were hybrids of the two species and their maternal parent was L. subspicata. The chemical composition of the root extract was examined for three of them as well as L. nelumbifolia and L. subspicata collected sympatrically. Nelumol was isolated from L. nelumbifolia, and furanoeremophilanes from L. subspicata. While nelumol and furanoeremophilanes were detected in two of the hybrid samples, only furanoeremophilanes were detected in the others.
1.Rikkyo Univ, Dept Life Sci, Toshima Ku, Tokyo 1718501, Japan 2.Rikkyo Univ, Res Ctr Life Sci, Toshima Ku, Tokyo 1718501, Japan 3.Ochanomizu Univ, Dept Chem, Bunkyo Ku, Tokyo 1128610, Japan 4.Rikkyo Univ, Dept Chem, Toshima Ku, Tokyo 1718501, Japan 5.Rikkyo Univ, Res Ctr Smart Mol, Toshima Ku, Tokyo 1718501, Japan 6.Forestry & Forest Prod Res Inst, Shikoku Res Ctr, Asakuranishi, Kochi 7808077, Japan 7.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China