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题名: ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1
作者: Li, Xing-Yu1; Mao, Zi-Chao1; Wang, Yue-Hu2; Wu, Yi-Xing1; He, Yue-Qiu1; Long, Chun-Lin2, 3
刊名: JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
出版日期: 2012
卷号: 22, 期号:2, 页码:83-93
关键词: Bacillus subtilis XF-1 ; Fengycins ; Antifungal fraction ; LC/ESI-MS/MS
DOI: 10.1159/000338530
文章类型: Article
英文摘要: Bacillus subtilis XF-1 (CGMCC No. 2357), a patent strain with good effects for controlling the clubroot of crucifer and many pathogenic fungi, was predicted to produce cyclic lipopeptide (CLP) antibiotics based on its genomic analysis. In this study, the CLPs were purified and determined with the following protocol: the supernatant of XF-1 cultivating mixture was firstly precipitated, then the precipitants were extracted with methanol and further separated by Sephadex LH-20 chromatography to test its antifungal activities. Fungi-inhibiting fractions were further characterized with LC/ESI-MS and LC/ESI-MS/MS. The results show that four molecular ion peaks [M+H](+) (m/z 1,464, 1,478, 1,492 and 1,506) from fungi suppression fraction were identified as fengycin A with fatty acid of C-16-C-19, fengycin B (C-14-C-17), fengycin C (C-15-C-18), fengycin D (C-15-C-18) and fengycin S (C-15-C-18). Fengycin C (C-15 and C-18), fengycin D (C-15, C-16 and C-18) and fengycin S (C-15, C-16 and C-18) were reported for the first time. The diversity of the fengycins that exist in this strain will help the elucidation of their biocontrol mechanisms. Copyright (C) 2012 S. Karger AG, Basel
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]: DESORPTION IONIZATION-TIME ; PEPTIDE ENZYME-INHIBITORS ; FLIGHT-MASS-SPECTROMETRY ; STRUCTURAL-CHARACTERIZATION ; AMYLOLIQUEFACIENS FZB42 ; RAPID IDENTIFICATION ; WHOLE-CELL ; FENGYCIN ; SURFACTIN ; GLOEOSPORIOIDES
收录类别: SCI
语种: 英语
WOS记录号: WOS:000306883100002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/20396
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
3.Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China

Recommended Citation:
Li, Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,et al. ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1[J]. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,2012,22(2):83-93.
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