ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1
Li, Xing-Yu1; Mao, Zi-Chao1; Wang, Yue-Hu2; Wu, Yi-Xing1; He, Yue-Qiu1; Long, Chun-Lin2,3
2012
发表期刊JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
卷号22期号:2页码:83-93
摘要Bacillus subtilis XF-1 (CGMCC No. 2357), a patent strain with good effects for controlling the clubroot of crucifer and many pathogenic fungi, was predicted to produce cyclic lipopeptide (CLP) antibiotics based on its genomic analysis. In this study, the CLPs were purified and determined with the following protocol: the supernatant of XF-1 cultivating mixture was firstly precipitated, then the precipitants were extracted with methanol and further separated by Sephadex LH-20 chromatography to test its antifungal activities. Fungi-inhibiting fractions were further characterized with LC/ESI-MS and LC/ESI-MS/MS. The results show that four molecular ion peaks [M+H](+) (m/z 1,464, 1,478, 1,492 and 1,506) from fungi suppression fraction were identified as fengycin A with fatty acid of C-16-C-19, fengycin B (C-14-C-17), fengycin C (C-15-C-18), fengycin D (C-15-C-18) and fengycin S (C-15-C-18). Fengycin C (C-15 and C-18), fengycin D (C-15, C-16 and C-18) and fengycin S (C-15, C-16 and C-18) were reported for the first time. The diversity of the fengycins that exist in this strain will help the elucidation of their biocontrol mechanisms. Copyright (C) 2012 S. Karger AG, Basel
关键词Bacillus Subtilis Xf-1 Fengycins Antifungal Fraction Lc/esi-ms/ms
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/20396
专题资源植物与生物技术所级重点实验室
作者单位1.Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
3.Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China
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GB/T 7714
Li, Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,et al. ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1[J]. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,2012,22(2):83-93.
APA Li, Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,Wu, Yi-Xing,He, Yue-Qiu,&Long, Chun-Lin.(2012).ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1.JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,22(2),83-93.
MLA Li, Xing-Yu,et al."ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1".JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY 22.2(2012):83-93.
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