| Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin | |
Suen, Yung Lee1; Tang, Hongmei2,3; Huang, Junchao2 ; Chen, Feng4; Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China.; huangjc@mail.kib.ac.cn; sfchencoe@pku.edu.cn
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| 2014-12-24 | |
| 发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 卷号 | 62期号:51页码:12392-12398 |
| 摘要 | Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites. |
| 关键词 | Astaxanthin Aurantiochytrium Fatty Acid Vitreoscilla Hemoglobin Thraustochytrids |
| 学科领域 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| DOI | 10.1021/jf5048578 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347138500015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/19376 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 通讯作者 | Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China.; huangjc@mail.kib.ac.cn; sfchencoe@pku.edu.cn |
| 作者单位 | 1.Univ Hong Kong,Pokfulam, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,et al. Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12392-12398. |
| APA | Suen, Yung Lee.,Tang, Hongmei.,Huang, Junchao.,Chen, Feng.,Huang,JC .,...&sfchencoe@pku.edu.cn.(2014).Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12392-12398. |
| MLA | Suen, Yung Lee,et al."Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12392-12398. |
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