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Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin
Suen, Yung Lee1; Tang, Hongmei2,3; Huang, Junchao2; Chen, Feng4; Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China.; huangjc@mail.kib.ac.cn; sfchencoe@pku.edu.cn
2014-12-24
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume62Issue:51Pages:12392-12398
AbstractDissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.
KeywordAstaxanthin Aurantiochytrium Fatty Acid Vitreoscilla Hemoglobin Thraustochytrids
Subject AreaAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
DOI10.1021/jf5048578
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000347138500015
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/19376
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorHuang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China.; huangjc@mail.kib.ac.cn; sfchencoe@pku.edu.cn
Affiliation1.Univ Hong Kong,Pokfulam, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,et al. Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12392-12398.
APA Suen, Yung Lee.,Tang, Hongmei.,Huang, Junchao.,Chen, Feng.,Huang,JC .,...&sfchencoe@pku.edu.cn.(2014).Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12392-12398.
MLA Suen, Yung Lee,et al."Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12392-12398.
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