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题名: Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin
作者: Suen, Yung Lee1; Tang, Hongmei2, 3; Huang, Junchao2; Chen, Feng4
刊名: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期: 2014-12-24
卷号: 62, 期号:51, 页码:12392-12398
关键词: astaxanthin ; Aurantiochytrium ; fatty acid ; Vitreoscilla hemoglobin ; thraustochytrids
学科分类: Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
DOI: 10.1021/jf5048578
通讯作者: Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China. ; huangjc@mail.kib.ac.cn ; sfchencoe@pku.edu.cn
文章类型: Article
英文摘要: Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.
类目[WOS]: Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]: Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]: GENE PHYLOGENY THRAUSTOCHYTRIACEAE ; TAXONOMIC REARRANGEMENT ; SCHIZOCHYTRIUM ; OPTIMIZATION ; XANTHOPHYLLS ; EMENDATION ; MORPHOLOGY ; ERECTION ; SQUALENE ; NOV
收录类别: SCI
项目资助者: 985 Project of Peking University ; 19 State Oceanic Administration of China ; "863" Plan of the Ministry of Science 20 and Technology of China [2012AA02A707]
语种: 英语
WOS记录号: WOS:000347138500015
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/19376
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Univ Hong Kong,Pokfulam, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
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