Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin
Suen, Yung Lee1; Tang, Hongmei2,3; Huang, Junchao2; Chen, Feng4; Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China.; huangjc@mail.kib.ac.cn; sfchencoe@pku.edu.cn
2014-12-24
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号62期号:51页码:12392-12398
摘要Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.
关键词Astaxanthin Aurantiochytrium Fatty Acid Vitreoscilla Hemoglobin Thraustochytrids
资助信息985 Project of Peking University; 19 State Oceanic Administration of China; "863" Plan of the Ministry of Science 20 and Technology of China [2012AA02A707]
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/19376
专题资源植物与生物技术所级重点实验室
通讯作者Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China.; huangjc@mail.kib.ac.cn; sfchencoe@pku.edu.cn
作者单位1.Univ Hong Kong,Pokfulam, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
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GB/T 7714
Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,et al. Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12392-12398.
APA Suen, Yung Lee.,Tang, Hongmei.,Huang, Junchao.,Chen, Feng.,Huang,JC .,...&sfchencoe@pku.edu.cn.(2014).Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12392-12398.
MLA Suen, Yung Lee,et al."Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12392-12398.
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